葫芦素B对肝癌细胞内阿霉素浓度的影响  

Effect of cucurbitacin B on concentration of doxorubicin in hepatocellular carcinoma cells

作　　者：杨姣[1,2] 周雪莹[1] 历文[1] 朱晓琳[1] 牛哲峰[1] 张玉玺[2] 李士峰[2] 张美侠[1] 

机构地区：[1]中国医科大学药学院临床药理教研室,沈阳110001 [2]黑龙江大庆油田总医院药学部,黑龙江大庆163001

出　　处：《实用药物与临床》2012年第6期347-350,共4页Practical Pharmacy and Clinical Remedies

基　　金：辽宁省自然科学基金资助项目(L2010565)

摘　　要：目的建立肝癌细胞H22和HepG2中阿霉素含量测定的高效液相色谱方法,以明确葫芦素B对阿霉素在细胞内浓度的影响。方法色谱柱为Waters Sunfine C18柱(4.6 mm×150 mm,5μm),流动相为甲醇-0.01%醋酸(40∶60,V/V),流速为1.0 mL/min,柱温为30℃,荧光检测激发波长为495 nm,发射波长为560 nm,以盐酸表阿霉素为内标。结果在HepG2细胞液中,阿霉素线性范围为0.1～6.4μg/mL(r=0.993 4,n=7),加样回收率为107.4%,RSD为3.77%;在H22细胞液中,阿霉素线性范围为0.625～20μg/mL(r=0.996,n=7),加样回收率为105.93%,RSD为5.64%。结论在肝癌细胞中,葫芦素B能明显促进阿霉素内流,减少外排。葫芦素B使肝癌细胞中阿霉素的浓度升高,两者联合可能起到更好的抗肝癌作用,葫芦素B可能逆转肝癌细胞的阿霉素耐药性。Objective To evaluate the effect of cucurbitacin（CuB） B on concentration of doxorubicin in hepatocellular carcinoma cells（HCC）.Methods The analysis was performed on HPLC.The column was Lichrospher C18（4.6 mm×150 mm,5 μm）,the mobile phase consisted of methanol-0.01% acetic acid solution（40∶ 60,V/V）at the flow rate of 1.0 mL/min.Epirubicin was used as the internal standard,and the excitation wavelength and emission wavelength were 495 nm and 560 nm respectively.The column temperature was 30 ℃.Results The linear range of doxorubicin in HepG2 was 0.1~6.4 μg/mL（r=0.993 4,n=7）.The average recovery rate was 107.4%,RSD was 3.77%.The linear range of doxorubicin in H22 was 0.625~20 μg/mL（r=0.996,n=7）.The average recovery rate was 105.93%,RSD was 5.64%.Conclusion CuB has significantly increased intracellular Dox concentration by promoting Dox influx and suppressing Dox efflux as well.It is suggested that combination of CuB with Dox may be a promising regime for chemotherapy of HCC.Furthermore,CuB might reverse the drug resistance of HCC.

关 键 词：葫芦素B 阿霉素 HEPG2 H22 HPLC 

分 类 号：R735.7[医药卫生—肿瘤]