原位分子杂交检测肺癌组织MRP基因表达及意义  

Expression and prognostic significance of multidrug resistance associated protein (MRP) gene in non-small cell lung cancer by in situ hybridization

作　　者：单根法[1] 钟竑[1] 张辅贤[1] 李国庆[1] 隆桂麟[1] 顾鹤定[1] 戚晓敏[1] 

机构地区：[1]上海第二医科大学附属新华医院成人心胸外科,200092

出　　处：《中华肿瘤杂志》2000年第1期27-29,共3页Chinese Journal of Oncology

基　　金：上海市自然科学基金!( 96ZB14 0 43 )

摘　　要：目的　研究多药耐药性相关蛋白基因 MRP基因 (multi drugresistanceassociatedproteingene ,MRPgene)对肺癌患者预后的影响。方法　应用地高辛抗体标记探针原位分子杂交结合免疫组化技术 ,检测 47例非小细胞肺癌 (NSCLC)根治术后石蜡组织标本中肺癌细胞MRP基因mRNA表达 ,并回顾性随访。结果　 47例组织标本中 ,肺癌细胞均有不同程度MRP基因mRNA过度表达 ,其表达水平与患者术后生存期、化疗疗效、复发及转移密切相关 ,与病理类型、肿瘤大小、淋巴结转移、病理分期、癌细胞分化程度、年龄、性别无明显相关性。结论　MRP基因与肺癌患者预后有密切相关性 ,检测MRP基因mRNA表达水平可作为预测肺癌患者预后、确定化疗方案的手段之一。Objective To study the effect of MRP gene overexpression on prognosis of patients with non small cell lung cancer (NSCLC).Methods Paraffin embedded tissues from 47 cases of NSCLC who had under gone radical tumor resection were examined for expression of MRP gene mRNA by in situ hybridization using labelled digoxigenin probes combined with immunohistochemistry. All the patients were retrospectively followed up.Results All of the 47 lung cancer specimens were found to have overexpression of MRP mRNA. It was significantly correlated with patients′ survival period, response to chemotherapy, recurrence and mestastases after surgery, but was not correlated with histology, tumor size, node status, TNM stage, degree of differentiation, age and sex.Conclusion Overexpression of MRP gene is a marker of prognostic significance in patients with NSCLC.

关 键 词：肺肿瘤 多药耐药性 MRP基因 预后 

分 类 号：R734.2[医药卫生—肿瘤]