不同方法检测乳腺癌患者体内CD44表达的分析  

Analysis of CD44 expression in patients with breast cancer with different methods

作　　者：彭士云[1] 郝慧婷[1] 郁向静[1] 

机构地区：[1]哈尔滨医科大学附属第三医院,哈尔滨150081

出　　处：《中国卫生检验杂志》2012年第6期1329-1331,共3页Chinese Journal of Health Laboratory Technology

基　　金：哈尔滨医科大学附属第三医院院内科研启动基金(JJ2009-12)

摘　　要：目的:用不同方法检测乳腺癌患者体内CD44的表达,对检测结果及其临床意义进行分析。方法:应用流式细胞术(FCM)检测乳腺癌组、乳腺良性疾病组及正常对照组外周血淋巴细胞(PBL)CD44的表达。用免疫组织化学技术(IHC)检测乳腺癌组织、良性疾病乳腺组织及正常乳腺组织中CD44蛋白的表达。结果:两种方法检测乳腺癌组CD44表达水平均明显高于乳腺良性疾病组及正常对照组,所得结果有一定相关性。乳腺癌患者术前PBL CD44的表达与乳腺癌组织中ER、PR的表达水平显著相关(P<0.05)。乳腺癌组CD44的表达与患者首胎年龄显著相关(P<0.05)。结论:FCM检测乳腺癌患者PBL CD44表达的检测方法,优于用IHC检测乳腺癌组织中CD44表达的传统方式,在乳腺癌早期诊断及病情监测方面有着较高的临床应用价值。Objective：To use different ways to detect CD44 contents in pantients with breast cancer,and investigate the clinical significance.Methods：The contents of CD44 were determined by flow cytometry（FCM） and immunohistochemistry（IHC） in patients with breast cancer,patients with benign breast diseases and normal controls.Results：The contents of CD44 were singnificantly higher in patients with breast cancer compared with those in patients with benign breast diseases and controls.The CD44 level of PBL was correlated with the expression of ER and PR in breast cancer patients,and the age of first gestation.Conclusion：The dection of FCM is better than ICH in CD44 determination.CD44 levels in PBL may be of important clinical value in diagnosis and evaluation for patients with breast cancer.

关 键 词：乳腺癌 CD44 免疫组织化学技术 流式细胞术 外周血淋巴细胞 

分 类 号：R737.9[医药卫生—肿瘤]