FISH检测HER-2基因在宫颈腺癌细胞中的表达及临床意义  

Expression and clinical significance of HER-2 gene in cervical scraping samples

作　　者：朱杰[1] 王攀[1] 张黎明[1] 陈巧佩[1] 李招云[1] 

机构地区：[1]浙江省台州市中心医院医学检验科,浙江台州318000

出　　处：《中国卫生检验杂志》2012年第6期1341-1343,共3页Chinese Journal of Health Laboratory Technology

基　　金：卫生部科学研究基金(WKJ2007-3-001)

摘　　要：目的:探讨HER-2基因在不同程度子宫颈病变脱落细胞中的表达以及临床意义。方法:采用荧光原位杂交技术(FISH)检测脱落细胞HER-2基因,其中宫颈腺癌(SCC)患者18例,上皮细胞内瘤变(CN)患者72例,正常细胞学妇女18例。结果:HER-2基因在正常对照、CNⅠ、CNⅡ/Ⅲ以及SCC患者中的阳性表达率分别为5.6%、10.2%、30.4%和50.0%。CNⅡ/Ⅲ、SCC患者与正常对照,HER-2基因阳性表达均有显著差别(P<0.05);SCC组与CNⅠ和CNⅡ/Ⅲ组比较,HER-2基因阳性表达差别也有统计学意义(P<0.05)。结论:HER-2基因高表达在子宫颈癌的发生、发展中可能起重要作用,FISH检测HER-2基因可以作为早期子宫颈癌筛查的方法之一。Objective：To investigate the expression and clinical significance of the HER-2 gene in cervicalscraping samples.Methods：The fluorescent in situ hybridization（FISH） was used to detect the expression of HER-2 gene in cervical scraping samples from squamous carcinomas of the cervix（SCC,n=18）,cervical intraepithelial neoplasia（CN.n = 72） and normal control cervix（n = 18）.Results：Positive expression rates of the HER-2 gene in normal control cervix,CNⅠ,CNⅡ/Ⅲ and SCC were 5.6%,10.2%,30.4%,50.0%.Compared with normal control cervix,HER-2 gene positive expression in CN Ⅱ/Ⅲ and SCC all had significant difference（P0.05）;There were also significant difference between SCC and CNⅠ,SCC and CNⅡ/Ⅲ（P0.05）.Conclusion：The over expression of HER-2 gene may play an important role in cervical carcinogenesis,therefore,it can be used as a better tumor index for early cervical cancer screening and accessory diagnosis.

关 键 词：HER-2基因 FISH技术 上皮细胞内瘤变 宫颈腺癌 

分 类 号：R730.43[医药卫生—肿瘤]