miR-195过表达促进人神经胶质瘤细胞T98G凋亡  

Overexpression of miR-195 promotes apoptosis in human glioma T98G cells

作　　者：赵志强[1] 阴彬[1] 彭小忠[1] 

机构地区：[1]中国医学科学院 基础医学研究所 北京协和医学院 基础学院 医学分子生物学国家重点实验室,北京100005

出　　处：《基础医学与临床》2012年第7期783-787,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(30825023)

摘　　要：目的检测过表达miR-195对人神经胶质瘤细胞T98G增殖的影响,探讨miRNA-195在胶质瘤发病过程中可能机制。方法用合成的miR-195模拟物,转染miR-195表达水平较低的人神经胶质瘤细胞T98G,用MTT、流式细胞术和TUNEL法分别检测T98G细胞增殖、细胞周期和凋亡,用Western blot检测细胞BCL-2的表达。结果 miR-195模拟物转染T98G后,成熟miR-195明显升高(P<0.05)。在T98G中过表达miR-195后,可明显抑制细胞增殖,过表达miR-195能促进T98G细胞的凋亡和下调细胞BCL-2的表达。结论过表达miR-195能显著促进T98G细胞凋亡,提示miR-195可能对胶质瘤的治疗起作用。Objective To investigate the influence of overexpression of miR-195 on cell proliferation in glioma T98G cells and explore the function of miR-195 in glioma. Methods T98G cells with lower expression of miR-195 were transfected with synthesized miR-195 mimics, then MTF assay, flow cytometry and TUNEL assay were performed to analyse the alterations of cellular phenotype, Western blot was performed to examine the expression level of BCL-2. Results Mature miR-195 increased significantly after miR-195 mimics transfection （P 〈 0. 05 ）. Overexpression of miR-195 induced significant cell proliferation suppression, and up-regulated miR-195 promoted apoptosis and reduced the expression of BCL-2 protein in T98G cells. Conclusions Overexpression of miR-195 significantly promotes apop- tosis of 3x）SG cells, suggesting that miR-195 may serve as a potential therapeutic agent for glioma.

关 键 词：miR-195 T98G细胞 细胞增殖 凋亡 BCL-2 

分 类 号：R739.4[医药卫生—肿瘤]