维甲酸诱导的神经管缺陷小鼠模型中神经系统相关基因的表达  

Expression of nervous system related gene in retinoic acid induced neural tube defects of mice

作　　者：周斌[1] 张靖[1] 潘艳芳[1] 阴彬[1] 彭小忠[1] 

机构地区：[1]中国医学科学院基础医学研究所,北京100005

出　　处：《基础医学与临床》2012年第7期814-818,共5页Basic and Clinical Medicine

摘　　要：目的研究在全反式维甲酸诱导小鼠神经管畸形模型中神经系统相关基因(ASH2L、HDAC4、NSPC1与DOK5)的表达变化。方法取8只E8.5的C57/BL6孕鼠随机均分为对照组和模型组。给对照组腹腔注射橄榄油,给模型组腹腔注射全反式维甲酸;E13.5取胚胎。用real-time PCR检测两组小鼠脑和脊髓中ASH2L、HDAC4、NSPC1与DOK5的mRNA表达;用Western blot检测蛋白表达。结果全反式维甲酸可诱导小鼠出现典型神经管畸形;与对照组相比,致畸胚胎脑和脊髓中,ASH2L、HDAC4、NSPC1和DOK5的mRNA和蛋白水平显著下降(P<0.05)。结论ASH2L、HDAC4、NSPC1和DOK5可能是抑制全反式维甲酸致神经管畸形的潜在基因。Objective To study the expression of four nervous system related genes： ASH2L, HDAC4, NSPC1 and DOK5 in the all-trans retinoic acid induced mouse neural tube defect model. Methods Divide eight E8.5 C57/ BL6 pregnant mice randomly into two groups： control group and experimental group. Control group was intraperito- neal injected by olive oil; the experimental group was intraperitoneal injected by all trans retinoic acid which was dissolved in olive oil; Embryos were taken at El3.5. The mRNA expression of ASH2L,HDAC4,NSPC1 and DOK5 in mouse brain and spinal cord was detected by real-time PCR; the protein expression was detected by Western blot. Results All trans retinoic acid can induce a typical neural tube defects in mice. Compared with the control group, the expression of mRNA and protein declined in fetal mice brain and spinal cord which is treated with all trans RA （P 〈0. 05）. Conclusions ASH2L,HDAC4,NSPC1 and DOK5 may be the underlying genes of inhibiting all trans RA induced neural tube defects.

关 键 词：全反式维甲酸 ASH2L HDAC4 NSPC1 DOK5 神经管畸形 

分 类 号：R-332[医药卫生]