脑梗死患者血浆HDL亚类组成及含量研究  

Distribution and concentration of high density lipoprotein subclasses in patients with brain infarction

作　　者：周永强[1] 田英[1] 张彩平[1] 王小鸥[2] 陈浩[2] 陈志军[1] 陈武哲[1] 龙石银[1] 

机构地区：[1]南华大学生物化学与分子生物学教研室,湖南衡阳421001 [2]南华大学附一医院

出　　处：《临床心血管病杂志》2012年第6期455-457,共3页Journal of Clinical Cardiology

基　　金：国家自然科学基金项目(No:30800474);湖南省科技厅项目(No:2010SK3037);湖南省教育厅重点项目(No:11A104);湖南省研究生创新项目(No:CX2010B385)

摘　　要：目的:分析脑梗死患者血浆高密度脂蛋白(HDL)亚类组成及含量。方法:采用双向电泳-免疫印迹检测法分析50例脑梗死患者和50例正常者HDL亚类的组成及含量。结果:与对照组比较,脑梗死患者血浆中preβ2-HDL和HDL2b含量显著降低(均P<0.01),小颗粒preβ1-HDL含量显著增加(P<0.01);患者血浆三酰甘油(TG)水平与preβ1-HDL、HDL3b、HDL2a和HDL2b含量呈显著负相关(均P<0.05),HDL-C、载脂蛋白AⅠ含量与preβ1-HDL、preβ2-HDL、HDL3c、HDL3b、HDL3a、HDL2a和HDL2b含量呈显著正相关(均P<0.05)。结论:脑梗死患者血浆大颗粒HDL含量明显降低,小颗粒HDL含量明显增加,提示脑梗死患者HDL代谢过程异常。Objective：To study the distribution and concentration of high density lipoprotein（HDL） subclasses in brain infarction patients. Method：Apolipoprotein concentrations of plasma HDL subspecies in 50 brain infarction patients and 50 normal controls were determined by two-dimensional gel electrophoresis associated with immunodection method. Result：Compared with normal controls,the concentration of preβ2-HDL and HDL2b were significantly lower,and small-sized preβ1-HDL was significantly higher in brain infarction patients（both P0.01）.Correlation between HDL subclasses and lipids in brain infarction showed that the levels of triglyceride（TG） were negatively correlated with preβ1-HDL,HDL3b,HDL2a and HDL2b（both P0.05）,the levels of apoAⅠ and HDL-C were positively correlated with preβ1-HDL,preβ2-HDL,HDL3c,HDL3b,HDL3a,HDL2a,HDL2b（both P0.05）. Result：Compared with normal controls,the concentration of preβ2-HDL and HDL2b were significantly lower,and small-sized preβ1-HDL was significantly higher in brain infarction patients（both P0.01）.Correlation between HDL subclasses and lipids in brain infarction showed that the levels of triglyceride（TG） were negatively correlated with preβ1-HDL,HDL3b,HDL2a and HDL2b（both P0.05）,the levels of apoAⅠ and HDL-C were positively correlated with preβ1-HDL,preβ2-HDL,HDL3c,HDL3b,HDL3a,HDL2a,HDL2b（both P0.05）. Conclusion：The particle size of HDL shifted towards smaller sizes,which indicates that HDL metabolism might be abnormal in brain infarction patients.

关 键 词：脑梗死 HDL亚类 双向电泳-免疫印迹法 

分 类 号：R743.33[医药卫生—神经病学与精神病学]