Rock2对肝癌细胞放疗敏感度的影响  

Effect of Rock2 on Radiation Sensitivity of Hepatocellular Carcinoma Cells

作　　者：刘天德[1] 余新[1] 刘秀霞[2] 袁荣发[1] 蒋成行[1] 邵江华[1,2] 

机构地区：[1]南昌大学第二附属医院肝胆外科,南昌330006 [2]江西省分子医学重点实验室

出　　处：《肿瘤防治研究》2012年第6期658-661,共4页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(30860272);江西省研究生创新基金资助项目(YC10A018)

摘　　要：目的探讨Rock2对肝癌细胞放疗敏感度的影响。方法降低人肝细胞癌Huh-7和HepG2细胞中Rock2的表达,利用IR照射造成DNA损伤模型,MTT法检测细胞增殖情况,Western blot检测Cdc25A的变化,并用能量共振转移法检测Cdk2/CyclinE活性的改变。结果 IR诱导DNA损伤时,干扰Rock2组细胞较Rock2正常组细胞增殖受到抑制。Western blot检测发现,降低Rock2表达可导致肝癌细胞中DNA损伤时Cdc25A表达的进一步下降,而且Cdk2/CyclinE活性也随Cdc25A的降低而降低。结论降低Rock2表达可能通过抑制Cdc25A而增强肝癌细胞的放疗敏感度,为肝癌的治疗及基因表达调控研究提供新的靶基因。Objective To investigate the effects of Rock2 on radiation sensitivity of hepatocellular carcinoma cells（HCC）.Methods The expression of Rock2 in HepG2 and Huh-7 cells was decreased and DNA damage model was constructed by IR exposure.MTT assay was used to detect cell proliferation.The change of Cdc25A expression was measured by Western blot and Cdk2/CyclinE activity was detected by fluorescence resonance energy transfer.Results shRock2 cells exposed to IR（to induce DNA damage） showed significantly decreased survival,compared with siControl cells.Western blot analysis revealed that when exposed to IR,Cdc25A decreased more obviously in shRock2 cells as compared to siControl cells.After exposure to IR,the decreased in Cdk2/Cyclin E activity was more apparent in shRock2 cells compared to the siControl cells.Conclusion Inhibition of Rock2 expression could enhance the radiation sensitivity of HCC by suppressing Cdc25A.Rock2 might be a new target gene for treatment of HCC.

关 键 词：ROCK2 肝细胞癌 CDC25A 放疗 

分 类 号：R735.7[医药卫生—肿瘤]