MMP-9和MMP-2基因多态性与原发性肝癌侵袭转移的关系  

Correlation of MMP-9 and MMP-2 Gene SNPs with Hepatocellular Carcinoma Invasion and Metastasis

作　　者：吴时胜 邵立华 李尚日[1] 张飞[1] 谢鸿[1] 张春秀[1] 刘桂平[1] 

机构地区：[1]黄石市第二医院消化内科,湖北黄石435002 [2]武汉市第五医院消化内科

出　　处：《肿瘤防治研究》2012年第6期683-686,共4页Cancer Research on Prevention and Treatment

摘　　要：目的探讨原发性肝癌中MMP-9和MMP-2基因多态性表达与原发性肝癌侵袭转移的关系。方法用聚合酶链反应—限制性片断长度多态性技术,检测MMP-2和MMP-9启动子基因型在28例原发性肝癌患者(其中8例有转移)和42例健康者中的频率。结果与携带MMP-9-1562CC和CT基因型相比,携带MMP-9-1562TT基因型者早期发生侵袭转移的风险增加1.25倍(95%CI:3.64～5.69),且这种风险增高与研究对象的年龄和性别无关,同时携带MMP-9-1562TT和MMP-2-1306CC或CT基因型的个体,患肝癌的风险性较单一携带的个体显著增高(P<0.5)。结论 MMP-2-1306T/C多态性单独与原发性肝癌风险无关,但与MMP-9-1562C/T多态性可能有基因-基因交互作用。MMP-2和MMP-9基因多态性与原发性肝癌侵袭转移可能相关。Objective To investigate the association between MMP-9 and MMP-2 gene SNPs of promoter regions and both infiltration and metastasis in hepatocellular carcinoma.Methods PCR-restriction fragment length polymorphism（PCR-RFLP） technique was applied to detect MMP-2 and MMP-9 promoter SNPs in 28 patients with hepatocellular carcinoma（8 cases with metastasis） and 42 healthy people.ResultsThe risk of early metastasis of MMP-9-1562TT genotype was increased up to 1.25-fold（95%CI,3.64~5.69）,compared with MMP-9-1562CC or CT genotype,and irrelevant to the age or gender of patients.The risk of liver cancer in population harboring MMP-9-1562TT and MMP-2-1306CC or CT genotypes was significantly higher than in those harboring MMP-9-1562TT,MMP-2-1306CC or TT alone.Conclusion MMP-2-1306T/C polymorphism alone is not a risk factor of primary liver cancer,although has synergistic interactions with MMP-9-1562C/T genotype,MMP-2 and MMP-9 gene polymorphism are related to the infiltration and metastasis of primary liver cancer.

关 键 词：原发性肝癌 MMP-2 MMP-9 基因多态性 

分 类 号：R735.7[医药卫生—肿瘤]