uPA基因启动子区低甲基化在鼻咽癌侵袭转移中的作用  

Promoter Hepomethylation of uPA Gene Play a Role in Invasion and Metastasis of Nasopharyngeal Cancer

作　　者：张松 李烁 高春生 

机构地区：[1]深圳市光明新区人民医院耳鼻喉科,广东深圳518106 [2]深圳市南山人民医院耳鼻喉科

出　　处：《肿瘤防治研究》2012年第6期691-693,共3页Cancer Research on Prevention and Treatment

基　　金：教育部博士学科点专项科研基金(新教师基金)(20070487172)

摘　　要：目的探讨uPA基因启动子区低甲基化状态与鼻咽癌侵袭转移的关系。方法用甲基化特异性PCR及RT-PCR法检测分析鼻咽癌及鼻咽部慢性炎症组织中uPA基因甲基化状态及其基因表达情况,分析uPA基因低甲基化与鼻咽癌侵袭转移的关系。结果 58例鼻咽癌组织中有51例为uPA基因低甲基化,其余7例为高甲基化,而鼻咽部慢性炎症组织中uPA基因均为高甲基化;低甲基化的鼻咽癌组织中均见uPA mRNA强表达,而高甲基化的鼻咽癌和鼻咽部慢性炎症组织中仅见uPA mRNA弱表达或无表达。结论鼻咽癌中uPA基因的高表达与其基因启动子区低甲基化有关,uPA基因低甲基化可能与鼻咽癌的侵袭转移有关。Objective To explore the relationship between of the promoter hepomethylation of uPA gene and invasion and metastasis of nasopharyngeal cancer.Methods Methylation-specific polymerase chain reaction（PCR） and reverse transcription PCR techniques were used to determine the promoter methylation status and mRNA expression of uPA gene in nasopharyngeal cancer and chronic inflammation tissue in pharynxnasalis.Results Hepomethylation of uPA gene promoter was found in 51 of 58 nasopharyngeal cancer samples.Hepermethylation of uPA gene promoter was found in the other seven nasopharyngeal cancer tissues.Hepermethylation of uPA gene promoter was found in all chronic inflammation tissue of pharynx nasalis.Over-expression of uPA mRNA was found in nasopharyngeal carcinoma tissues which promoter hepomethylation.Weak or no expression of uPA mRNA was found in nasopharyngeal carcinoma which promoter hepermethylation cance and chronic inflammation tissue in pharynx nasalis.Conclusion Over-expression of uPA gene is relate to promoter hepomethylation in nasopharyngeal cancer tissues.Promoter hepomethylation of uPA gene possiblely concerned with invasion and metastasis of nasopharyngeal cancer.

关 键 词：尿激酶型纤溶酶原激活剂(uPA) 低甲基化 鼻咽癌 

分 类 号：R739.6[医药卫生—肿瘤]