9-顺式维甲酸抑制甲状腺鳞癌细胞SW579增殖的作用机制  

Mechanism of 9-cisRA in Inhibiting the Proliferation of Thyroid Squamous Cell Carcinoma Cell SW579

作　　者：张秀梅[1] 刘超[1] 赵颂[1] 王翠瑶[1] 肖建英[1] 

机构地区：[1]辽宁医学院生物化学教研室,辽宁省锦州市121000

出　　处：《中国肿瘤临床》2012年第11期761-764,共4页Chinese Journal of Clinical Oncology

基　　金：辽宁省教育厅科研项目(编号:LS2010101)资助~~

摘　　要：目的:观察9-顺式维甲酸(9-cis-retinoic acid,9-cisRA)对甲状腺鳞癌细胞株SW579细胞RARβ、p21、CDK4、CyclinDl表达的影响,进而探讨9-cisRA的抗癌作用机制方法:分别以终浓度为1×10^(-7)、1×10^(-6)、5×10^(-6)、1×10^(-5)mol/L 9-cisRA作用SW579细胞,各组细胞均在培养24 h后加药,继续培养48 h。用RT-PCR方法检测SW579细胞RARβ、p21、CDK4、CyclinD1的mRNA表达;用Western Blot检测SW579细胞RARβ、p21、CDK4、CvclinD1的蛋白表达结果:9-cisRA作用后,RT-PCR检测结果表明,经不同浓度9-cisRA处理的细胞RARB、p21的mRNA表达水平均显著上调;CDK4的mRNA表达水平无明显变化;CyclinD1的表达水平明显下调;Western Blot检测结果表明经不同浓度9-cisRA处理的细胞RARβ、p21蛋白表达水平均显著上调;CDK4蛋白表达水平无明显变化;CyclinD1蛋白表达水平明显下调。结论:9-cisRA在一定浓度范围内可能通过上调RARβ、p21的表达进而下调细胞周期蛋白CvclinD1的表达,从而抑制细胞增殖。Objectives： The current study aims to observe the effect of 9-cisRA in the expressions of RARJ3, p21, CDK4, and CyinD1 genes in the thyroid squamous cell carcinoma cells （ SW579 ） to explore its mechanism. Methods： The SW579 ceils were treated with different doses of 9-cisRA （ final concentration of 1×10^（-7） mol/L, 1×10^（-6） tool/L, 5×10^（-6） tool/L, and 1×10^（-5） mol/L ）. The drug was added in the cells of all groups 24 h after the culture. The cells were then cultured for another 48 h. The RARβ, p21, CDK4, and CyclinD1 mRNA expression levels were determined via RT-PCR, whereas the expression of RARβ, p21, CDK4, and CyclinD1 protein were determined using Western blot. Results： The mRNA expression of RARβ and p21 increased dramatically after the cells were treated with 9-cisRA, whereas the expression of CDK4 did not increase. In addition, the mRNA expression of CyclinD1 dramatically decreased, whereas the protein expression of RARβ and p21 significantly increased. No distinct change in the protein expression of CDK4 was observed. The CyclinD 1 protein expression was dramatically reduced. Conclusion： The drug 9-cisRA can inhibit the proliferation of thyroid squamous cell carcinoma cells in a dose-dependent manner. The mechanism of the effect may be related to the increase of RARβ and p21mRNA and protein expression, as well as the decrease of CyclinD 1 mRNA and protein expression.

关 键 词：9-顺式维甲酸 SW579 RARβ P21 CDK4 CYCLIND1 

分 类 号：R736.1[医药卫生—肿瘤]