腹腔持续注射血管紧张素-(1-7)对胆管结扎大鼠肝纤维化的抑制作用  

Prolonged angiotensin-(1-7) infusion inhibits hepatic fibrosis in rats with bile duct ligation

作　　者：李旭[1] 宁佐伟[1] 罗薇[2] 张文雍[1] 余常辉[3] 孟莹[3] 

机构地区：[1]南方医科大学南方医院急诊科消化科,广东广州510515 [2]南方医科大学南方医院消化科,广东广州510515 [3]南方医科大学南方医院呼吸科,广东广州510515

出　　处：《南方医科大学学报》2012年第7期944-947,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30770974,30871155)~~

摘　　要：目的探讨血管紧张素(1-7)[Ang-(1-7)]对胆管结扎(BDL)大鼠肝纤维化的抑制作用。方法 18只Wister雄性大鼠随机分为假手术(SHAME组)组,BDL组和Ang-(1-7)治疗组。假手术组:行开腹术再关腹,在腹腔埋注射泵,以25μg.kg-1.h-1的速度持续注射生理盐水;BDL组:开腹结扎胆总管建立肝纤维化模型,在腹腔埋注射泵,以25μg.kg-1.h-1的速度持续注射生理盐水;Ang-(1-7)治疗组:BDL建立肝纤维化模型,同时在腹腔埋注射泵,以25μg.kg-1.h-1的速度持续注射Ang-(1-7)。4周后宰杀动物、取肝组织,行Masson胶原染色,肝纤维化评分,测定羟脯氨酸含量,免疫组织化学检测α-肌动蛋白(α-SMA)的表达。结果与BDL组相比,Ang-(1-7)治疗组肝纤维化评分(2.33±0.52 vs 5.17±0.75)、胶原面积(23.71±3.43 vs 89.77±9.92)、羟脯氨酸含量(0.36±0.03 vs 0.52±0.04)、α-SMA的表达(54.11±17.55 vs 191.84±31.72)均减少,有统计学意义(P<0.01)。结论Ang-(1-7)对胆总管结扎所致的肝纤维化具有抑制作用。Objective To observe the inhibitory effect of angiotensin-（1-7） on liver fibrosis induced by bile duct ligation in rats. Methods Eighteen Wistar rats were randomized into 3 groups and subject to sham operation, bile duct ligation （BDL）, or BDL with angiotensin-（1-7） treatment. An osmotic minipump was implanted intraperitoneally for administration of saline in the sham-operated and BDL groups and angiotensin-（1-7） （25ug. kg-1. h-1） in angiotensin-（1-7） treatment group. After a 4-week treatments, the fibrosis score, Masson staining, and hydroxyproline assay were used to evaluate the level of liver fibrosis in the rats, and immunohistochemistry was used to detect expression of a-smooth muscle actin （a-SMA） in the liver tissue. Results Compared with BDL group, a 4-week treatment with angiotensin-（1-7） following BDL significantly reduced the fibrosis score （2.33±0.52 vs 5.17±0.75）, hydroxyproline content （0.30±0.03 vs 0.52±0.04） and a-SMA expression （54.11±17.55 vs 191.84±31.72） in the liver tissue of the rats （P〈0.01）. Conclusion Prolonged infusion of angiotensin-（1-7） inhibit the formation of hepatic fibrosis in rats following bile duct ligation.

关 键 词：血管紧张素-(1—7) 肝纤维化 Α-肌动蛋白 胆管结扎 

分 类 号：R575.2[医药卫生—消化系统]