CASP8在鼻咽癌中的表达及临床意义  

Reduced expression of CASP8 in nasopharyngeal carcinoma and its clinical significance

作　　者：方唯意[1] 张千兵[1] 刘真[1,2] 江庆萍[3] 

机构地区：[1]南方医科大学肿瘤研究所,广东广州510515 [2]广州医学院病理教研室,广东广州510182 [3]广州医学院第三附属医院病理科,广东广州510150

出　　处：《南方医科大学学报》2012年第7期963-965,969,共4页Journal of Southern Medical University

基　　金：国家自然科学基金委和广东省联合重点基金(U0732006);广州市珠江科技新星计划(2011J2200009);广东省育苗工程计划(LYM11102)~~

摘　　要：目的探讨CASP8在鼻咽癌中的表达及临床意义。方法利用基因芯片比较了8组鼻咽癌组织与混合的非癌鼻咽组织之间的差异表达基因。荧光定量PCR和免疫组化鉴定CASP8基因表达结果的可靠性,并分析CASP8在鼻咽癌中的表达与临床参数之间的相关性。结果荧光定量PCR确证CASP8 mRNA在鼻咽癌组织中表达下调(P<0.0001),与芯片结果相一致。免疫组化结果显示,与非癌鼻咽组织相比,CASP8蛋白在鼻咽癌组织中表达明显下调(P=0.02),且下调的表达与淋巴结转移(P=0.002)及临床分期(P=0.026)呈负相关。结论 CASP8表达下调作为不利因素促进了鼻咽癌的发生发展。Objective To explore the expression of CASP8 and its clinical significance in nasopharyngeal carcinoma （NPC）. Methods The differentially expressed genes between pooled NPC tissues and non-cancerous nasopharyngeal （NP） tissues were screened using 8 microarrays. Real-time PCR and immunohistochemistry were used to validate the detection results of CASP8 expression in NPC, and the correlation of CASP8 expression to the clinical characteristics was analyzed in the NPC cases. Results Real-time PCR confirmed a reduced expression of CASF8 mRNA level in NPC tissues （P〈0.0001）, which was consistent with the microarray data. Immunohistochemistry indicated that CASP8 protein expression was also significantly down-regulated in NPC tissue compared to that in the non-cancerous nasopharyngeal tissues （P=0.02）. The reduction of CASP8 expression was inversely correlated to lymph node metastasis （P=0.002） and the clinical stages （P=0.026） of NPC. Conclusion Decreased CASP8 expression is an unfavorable factor that promotes the development and progression of NPC.

关 键 词：鼻咽癌 基因芯片 CASP8 免疫组化 

分 类 号：R739.63[医药卫生—肿瘤]