巨细胞病毒感染对重型β-地中海贫血儿童异基因造血干细胞移植后早期T细胞亚群的影响  

Impact of cytomegalovirus infections on T lymphocyte subsets in children with β-thalassemia major early after allogeneic hematopoietic stem cell transplantation

作　　者：王彦华[1] 吴学东[1] 冯晓勤[1] 何岳林[1] 裴夫瑜[1] 李春富[1] 

机构地区：[1]南方医科大学南方医院儿科,广东广州510515

出　　处：《南方医科大学学报》2012年第7期1008-1011,共4页Journal of Southern Medical University

基　　金：广东省科技计划项目(2011B031800280)

摘　　要：目的探讨巨细胞病毒(CMV)对儿童重型β-地中海贫血(地贫)儿童异基因造血干细胞移植(Allo-HSCT)后180 d外周血T细胞亚群的变化。方法对我院儿科造血干细胞移植中心2010年1月～2011年1月儿童重型地贫Allo-HSCT 35例病例半年后的T细胞亚群进行分析,比较有CMV(13例)和无CMV感染组(22例)在造血干细胞移植后早期T细胞的变化。结果半年内有CMV组CD8+比率高于无CMV感染组,但CD4+比率低。在CMV感染组伴随CD8+CD28+细胞比率降低,CD8+CD28-细胞比率和CD8+比率升高,同时发现CD8+与CD8+CD28-细胞比率呈线性相关性。结论重型β-地中海贫血儿童在异基因造血干细胞移植后,CMV感染后会导致CD8+CD28-细胞堆积,是构成CD8+细胞升高的主因,且两者比率呈线性相关。Objective To investigate the effect of human cytomegalovirus （HCMV） infection on T lymphocyte subsets in children with β-thalassemia major （TM） during the initial 6 months after allogeneic hematopoietic stem cell transplantation （Allo-HSCT）. Methods From January, 2010 to January, 2011, 35 children with TM underwent Allo-HSCT. Peripheral blood samples were obtained from the children 6 month after the transplantation to examine the changes of T lymphocytes subsets in relation to HCMV seropositivity. Results Thirteen children were found seropositive and 22 were seronegative for HCMV. The HCMV-seropositive children had a higher CD8＋ cell percentage but a lower CD4＋ cell percentage than those without HCMV infection. Compared with those seronegative for HCMV, the children with HCMV seropositivity showed increased percentages of CD8＋ ceils and CD8＋CD28 cells with a decreased percentage of CD8＋CD28＊ cells. A positive linear correlation was found between the percentages of CD8＋ CD28 cells and CD8＋ cells. Conclusion HCMV infection can lead to the accumulation of CD8＋CD28 cells to cause increased CD8＋ T cells in the peripheral blood in TM children after Allo-HSCT. The percentages of CDS＋CD28- cells has a positive linear correlation to that of CD8＋ cells.

关 键 词：巨细胞病毒 Β-地中海贫血 儿童 造血干细胞移植 T细胞亚群 

分 类 号：R725.5[医药卫生—儿科]