C-IAP2在肝细胞肝癌中的表达及与临床预后的关系  

Association of cellular inhibitor of apoptosis protein-2 expression with clinical outcomes of hepatocellular carcinoma

作　　者：毕民平[1,2] 杨定华[1] 李湘竑[1] 钟克波[1] 刘延[1] 李光辉[1] 廖晓明[1] 刘萧[1] 

机构地区：[1]南方医科大学南方医院肝胆外科,广东广州510515 [2]新乡市中心医院普瘤外科,河南新乡453000

出　　处：《南方医科大学学报》2012年第7期1020-1025,1030,共7页Journal of Southern Medical University

基　　金：广东省自然科学基金(10151051501000120);广东省教育厅人才引进基金

摘　　要：目的探讨C-IAP2 mRNA和蛋白在肝细胞癌中的表达及其与临床预后的关系。方法采用荧光定量PCR和免疫组化方法检测肝细胞癌组织及对应的非癌组织中C-IAP2 mRNA和蛋白的表达水平并分析肝癌组织中C-IAP2的表达和临床病理特征之间的关系。结果肝细胞肝癌中C-IAP2 mRNA的表达是非癌组织的2.70倍(P<0.001)。C-IAP2蛋白在肝癌组织阳性表达率为70.8%,显著高于非癌组织中的阳性表达率27.8%(P=0.001)。C-IAP2 mRNA和蛋白在肝癌组织中的表达与术前有无癌栓、有无淋巴结转移、甲胎蛋白水平、肿瘤病理分化类型、TNM分期以及术后复发转移时间有关(P<0.05),而与患者性别、年龄、乙型肝炎表面抗原、肿瘤大小、肿瘤数目、肝硬化以及包膜完整性无关(P>0.05)。结论 C-IAP2表达水平与原发性肝癌的转移、复发有关,可作为原发性肝细胞癌预后的新生物学指标。Objective To study the expression of cellular inhibitor of apoptosis protein-2 （C-IAP2） mRNA and protein in hepatocellular carcinoma （HCC） and its relationship with the clinical outcomes. Methods Quantitative PCR and immunohistochemical staining were used to detect the expression of C-IAP2 mRNA and protein in the tumor tissues and corresponding adjacent non-cancerous tissues from HCC patients. Results The expression of C-IAP2 mRNA in HCC tissues was 2.70 folds higher than that in the non-cancerous tissues （P〈0.001）. The expression rate of C-IAP2 protein in HCC tissues was 70.8%, significantly higher than that in the non-cancerous tissues （27.8%, P=0.001）. The expression of C-IAP2 mRNA and protein was associated with the tumor emboli, lymph node metastasis, AFP level, histological differentiation, TNM stage, postoperative recurrence and metastasis （P〈0.05）, but not with the patients＇ gender, age, HbsAg positivity, number of tumors, cirrhosis or the presence of tumor encapsulation （P〉0.05）. Conclusion The expression of C-IAP2 in HCC is associated with tumor recurrence and metastasis, and can be a biological marker for prognostic evaluation of HCC.

关 键 词：细胞凋亡抑制蛋白2 肝细胞肝癌 荧光定量PCR 免疫组化 

分 类 号：R735.7[医药卫生—肿瘤]