柴芪益肝颗粒联合紫杉醇抑制裸鼠肝癌生长的在体成像研究  

Chaiqiyigan granula enhances Taxol-induced growth inhibition of hepatocellular carcinoma xenografts in nude mice:an in vivo fluorescence imaging study

作　　者：游敏玲 罗满芳 廖蔚茜 胡世平 许文学[2] 靖林林[2] 

机构地区：[1]罗湖区中医院一病区,广东深圳518000 [2]南方医科大学中西医结合医院,广东广州510315

出　　处：《南方医科大学学报》2012年第7期1042-1045,共4页Journal of Southern Medical University

基　　金：深圳市罗湖区科技计划项目(200923)

摘　　要：目的探讨柴芪益肝颗粒(CG)和紫杉醇(taxol)对裸鼠异位肝癌的作用及对Bax、p53和VEGF表达的影响。方法应用转染及G418筛选构建单克隆Hep G2/EGFP细胞系,细胞注射至裸鼠形成异位肝癌模型,应用荧光成像技术在体观察在柴芪益肝颗粒合Taxol作用下肿瘤的生长,用免疫组化法检测肿瘤组织中Bax、p53、VEGF的表达。结果与对照组相比,Taxol组和Taxol+CG组在3周和4周时降低肝癌增殖速度,Taxol+CG组抑制作用显著优于Taxol组比。Taxol+CG组增加Bax表达,降低p53表达和VEGF表达。结论柴芪益肝颗粒明显增强Taxol对裸鼠异位肝癌生长的抑制作用,其作用与增加Bax表达、抑制p53、VEGF表达相关。Objective To study the effects of Chaiqiyigan Granula （CG） and Taxol on the growth of hepatoceUular carcinoma （HCC） xenografts and expression of Bax, p53 and VEGF in nude mice. Methods Whole-body fluorescence imaging was used to visualize the growth of HCC in nude mice bearing hepG2/EGFP cell xenograft. Immunohistochemistry was used to determine the content of Bax, p53 and vascular endothelial growth factor （VEGF） in the tumor tissues. Results Compared with normal saline, Taxol alone and in combination with CG significantly inhibited the growth of HCC xenografts in the nude mice. The combined treatment with CG and Taxol produced a stronger inhibitory effect on the tumor growth than Taxol alone in the third and fourth weeks. The volume and weight of the xenografts were decreased in the combined treatment group compared with those in saline treatment group. CG combined with Taxol increased the expression of Bax and reduced the expression of p53 and VEGF in the tumor xenografts. Conclusion CG can enhance the inhibitory effects of Taxol on the growth of HCC xenografts, and this effect is related to the up-regulation of Bax and down-regulation of p53 and VEGF expression in the tumor.

关 键 词：柴芪益肝颗粒 紫杉醇 活体荧光成像 BAX p53 VEGF 

分 类 号：R735.7[医药卫生—肿瘤] R273[医药卫生—临床医学]