胰腺癌细胞Panc-1中AKT2基因表达对吉西他滨敏感性的影响  

Effects of AKT2 expression on gemcitabine sensitivity in pancreatic cancer cell line Panc-1

作　　者：彭涛[1] 周峰[1] 杨鹏[1] 王春友[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胰腺外科,武汉430022

出　　处：《中华肝胆外科杂志》2012年第6期459-461,共3页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的探讨胰腺癌细胞Panc-1中AKT2基因表达对吉西他滨敏感性的影响。方法体外将AKT2特异性shRNA表达载体pAKT2-shRNA转染胰癌细胞株Panc-1。应用RT—PCR、Westernblot检测转染shRNA后Pane-1细胞AKT2基因和蛋白的表达变化，应用MTT法检测干扰AKT2后Pane-1细胞对吉西他滨敏感性的变化。结果转染pAKT2～shRNA后Pane-1细胞AKT2基因和蛋白的表达水平明显下降；吉西他滨对Pane-1细胞的半数抑制量从（1．96±0．22）mg／L降到（O．24±0．03）mg／L，Panc-1细胞对吉西他滨的敏感性明显增加。结论用pAKT2-shRNA抑制AKT2基因表达，能增加人胰癌细胞株Panc-1对吉西他滨的敏感性。Objective To explore the effects of AKT2 expression in pancreatic cancer cell line Panc-1 on the sensitivity towards to gemcitabine. Methods The expression vector pAKT2-shRNA was constructed and transfected into Panc-1 cells by lipofecton. The mRNA and protein expression levels of AKT2 were detected by RT-PCR and Western blot assays, respectively. The changes of gem- citabine sensitivity after shRNA were examined by the MTT assay. Results The mRNA and protein levels of AKT2 in Panc-1 cells were significantly decreased after the transfection, and the median inhi- bition concentration of gemcitabine against Panc-1 cells was reduced from （1.96±0.22） mg/L to （0.24±0.03） mg/L. The sensitivity of Panc-1 cells to gemcitabine was increased significantly after pAKT2-shRNA transfeetion. Conclusion The sensitivity of Panc-1 cells to gemcitabine could be en- hanced by pAKT2-shRNA.

关 键 词：胰腺癌 基因 吉西他滨 

分 类 号：R735.9[医药卫生—肿瘤]