哺乳期2,3,7,8-TCDD暴露仔鼠牙齿釉质的矿物质含量和晶体特性分析  

Effects of Lactational 2,3,7,8-TCDD Exposure to Mineral Content and Cry Stallo-graphic Properties in Tooth Enamel of SD Rat Offspring Rats

作　　者：耿华欧[1] 章锦才[1] 东野广智[2] 王京滨[3] 

机构地区：[1]广东省口腔医院牙周科,广东广州510280 [2]广东医学院分析中心 [3]广东省疾病预防控制中心

出　　处：《口腔医学研究》2012年第6期519-521,共3页Journal of Oral Science Research

基　　金：广东省卫生厅医学科研基金立项课题(编号:A2010083)

摘　　要：目的:探讨哺乳期内2,3,7,8-TCDD暴露对牙齿釉质的矿物质含量和晶体特性的影响。方法:分别从2,3,7,8-TCDD暴露组和对照组PD60仔鼠的牙齿釉质提取样品。应用组织灰化法测定矿物质含量。应用红外光谱技术评价晶体性质。结果:2,3,7,8-TCDD暴露组牙齿釉质矿物质含量同对照组相比出现降低情况,2组间比较,差异明显(分泌-早期成熟期釉质:57.8±8.9Vs 42.2±10.8,P<0.01;成熟期釉质:90.3±15.2Vs 75.4±18.5,P<0.05)。所有釉质样品中均出现CO32-和PO43-特征性吸收峰,2,3,7,8-TCDD暴露组CO32-吸收峰吸光度较对照组高。红外吸收峰的峰形显示及定量测定显示,对照组釉质的CO32-∶PO43-吸光度比较2,3,7,8-TCDD暴露组降低(分泌-早期成熟期釉质:0.4170±0.0324Vs 0.4726±0.0533,P<0.01;成熟期釉质:0.3356±0.0427Vs 0.3944±0.0538,P<0.01)。结论:2,3,7,8-TCDD暴露使牙齿釉质的矿物质含量降低,牙齿釉质晶体结构出现改变。Objective：To explore the relationship between lactational TCDD-exposure and the changes of dental enamel mineral content and crystallographic properties in rats.Methods：Enamel samples were obtained from TCDD-exposure group and control group.The incinerated tissue method was used to measure mineral content.The Fourier Transform Infrared spectroscopy was used to assess the crystallographic properties.Results：The mineral content in TCDD-exposed group shows a downwards trend compared to control group.Differences between two groups reach significance（secrectory-early mature enamel：57.8±8.9vs 42.2 ±10.8,P〈0.01;mature enamel： 90.3±15.2vs 75.4±18.5,P〈0.05）.There were characteristic absorbency peak of CO32-and PO43-in all enamel samples,and the peak of CO32-in 2,3,7,8-TCDD group was higher than the control.The display of peak and quantitative assay,the absorbency of CO32-/PO43-in control group decreased compared to the 2,3,7,8-TCDD group（secrectory-early mature enamel：0.4170±0.0324vs 0.4726 ±0.0533,P〈0.01;mature enamel：0.3356± 0.0427vs 0.3944±0.0538,P〈0.01）.The ratio of structural carbonate to phosphate in dental enamel of control group is significantly higher than 2,3,7,8-TCDD group.Conclusion：These results indicate that in TCDD-exposed group mineral content decreased and crystallographic properties of enamel changed.

关 键 词：哺乳期暴露 二恶英 矿物质含量 晶体特性 

分 类 号：R780.2[医药卫生—口腔医学]