银叶树树叶中总黄酮提取工艺优化  

Optimization of Extraction on Flavonoids from Leaves of Heritiera littoralis

作　　者：张艳军[1] 彭重威[1] 徐淑庆[1] 郑韵英[1] 谢清娟[1] 

机构地区：[1]钦州学院,广西钦州535000

出　　处：《中药材》2012年第4期638-641,共4页Journal of Chinese Medicinal Materials

基　　金：广西教育厅项目资助(200911LX437);广西自然科学基金项目(2011GXNSFA018123)

摘　　要：目的:优化工艺条件,从红树林植物银叶树Heritiera littoralis Dryand.树叶中提取具有抗氧化、抗癌等活性的黄酮类化合物。方法:将微波浸提法和超声波浸提法进行比较,紫外可见分光光度法在510 nm处对提取液中黄酮含量进行测定,考察提取时间、提取功率、溶剂浓度、料液比四个单因素对黄酮提取率的影响。根据L9(34)正交试验对黄酮的提取条件进行优化。结果:确定溶剂提取黄酮的工艺参数为:采取微波法提取,处理时间为8 min,微波提取功率400 W,乙醇浓度70%,料液比(g∶mL)1∶30,该条件下黄酮提取率达6.15%。结论:利用银叶树提取黄酮具有开发利用价值,为红树林植物药用成分的进一步开发研究奠定基础。Objective ： To optimize the preparation procedure for extraction of flavonoids from leaves of Heritiera littoralis. Methods ： Comparing microwave extraction and ultrasonic extraction, The content of total flavonoids was measure by UV spectrophometry and the wavelength set at 510 nm. The effects of treatment time, extraction power, ethanol concentration and solid-liquid ratio were studied. The orthogonal test of four factors and three levels were studied to obtain the extraction technology of flavonoids from leaves of Heritiera littoralis. Results：The optimal technical conditions were mixing leavesof Heritiera littoralis with 70% ethanol on the ratio of 1 g： 30 mL, when microwave treatment time was 8 min and micro-wave power was 400 W. Under this condition, the extraction rate reached 6. 15%. Conclusion ：This study explores a practical extraction method for flavonoids from leaves of Heritiera littoralis, and lay a good foundation for mangrove medical research in future.

关 键 词：银叶树树叶 黄酮类化合物 微波提取 超声波提取 

分 类 号：R914.4[医药卫生—药物化学]