电压门控质子通道Hv1对脑胶质瘤细胞SHG-44的迁移和侵袭的影响  

Effects of Voltage-gated Proton Channel Hv1 on Migration and Invasion of Glioma Cell SHG-44

作　　者：王汉中[1,2] 吴兴业[3] 王一凡[2] 纪志娜[2] 赵振波[1] 李树杰[2] 

机构地区：[1]南开大学物理科学学院,天津300071 [2]长春工业大学化学与生命科学学院,吉林长春130012 [3]通化市中心医院病理科,吉林通化134000

出　　处：《南开大学学报（自然科学版）》2012年第2期12-16,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金(30840028;30970579);天津市应用基础及前沿技术研究计划(08JCYBJC25800);教育部博士点基金(200800551035)

摘　　要：电压门控质子通道Hv1具有调节细胞内pH值的功能,用RT-PCR、细胞划痕和体外侵袭实验研究Hv1对脑胶质瘤细胞SHG-44迁移和侵袭的影响.RT-PCR结果表明,Hv1在SHG-44中表达;用针对Hv1基因的小RNA干扰片段(SiRNA)下调Hv1基因在SHG-44细胞中的表达明显减弱细胞的迁移和侵袭能力.明胶酶谱实验结果证明,下调Hv1基因在SHG-44细胞中的表达抑制了MMP-2、MMP-9的活性.以上结果表明,Hv1通过调节SHG-44细胞内的pH值来提高SHG-44细胞的迁移和侵袭能力.Voltage-gated proton channel （Hvl） can regulate intracellular pH value. The effects of Hvl gene on the migration and invasion of glioma cell line SHG-44 using RT-PCR, scratch and Matrigel invasion methods were studied. The results showed that Hv1 is expressed in SHG-44 by RT-PCR. The migration and invasion of glioma cells were significantly attenuated when Hvl was knocked down by SiRNA-Hv1. The results of eymographic analysis of matrix metalloproteinase indicated that the enzyme activities of MMP-9 and MMP-2 were attenu- ated when the reduction of Hvl expression in SHG-44 by SiRNA-Hv1. The results showed that Hvl promotes the migration and invasion of SHG-44 through regulating the intracellular pH value.

关 键 词：电压门控质子通道 脑胶质瘤 SIRNA 迁移 侵袭 

分 类 号：R739.4[医药卫生—肿瘤]