靶向polo样激酶-1基因小干扰RNA质粒的构建及其效能检测  

Construction of PLK1 siRNA-express plasmid and detection of its effects

作　　者：倪侃[1] 陈佳慧[1] 贾乃昕[1] 吴龙祥[1] 常仁安[1] 陈钟[1] 

机构地区：[1]南通大学附属医院普外科,南通226001

出　　处：《南通大学学报（医学版）》2012年第3期183-184,F0002,共3页Journal of Nantong University(Medical sciences)

基　　金：南通市社会发展基金资助项目(S2009031)

摘　　要：目的:用脂质体法构建polo样激酶-1(polo-like kinase-1,PLK1)基因RNA(siRNA)。方法 :设计合成PLK1siRNA干扰序列,构建干扰载体,然后以该siRNA转染SMMC-7721细胞后,以实时定量Western Blot检测各组细胞PLK1蛋白表达情况。结果:应用靶向PLK1的siRNA转染SMMC-7721细胞48 h后,PLK1蛋白的表达siRNA分别较无关对照组和空白对照组有明显下降。结论:通过脂质体转染法成功构建靶向PLK1的siRNA,实现了细胞的稳定转染,为进一步研究奠定了基础。Objective： To construct polo-like kinase-1（PLK1） small interfering RNA（siRNA） using liposomes.Methods： A recombinant plasmid containing antisense RNA targeting PLK1 was transfected into SMMC-7721 cells by liposomes.Western Blot was used to detect the PLK1 protein expression in SMMC-7721 cells,which was transfected with PLK1 siRNA.Results： Compared with blank control and negative control 48 h after transfection,siRNA reduced PLK1 protein significantly.Conclusion： PLK1 siRNA was successfully constructed by liposomes,which could achieve stable transfection.It provides a foundation for the future research.

关 键 词：肝细胞性肝癌 polo样激酶-1 小干扰RNA 

分 类 号：R735.7[医药卫生—肿瘤]