白藜芦醇对人胃癌SGC-7901细胞形态、线粒体膜电位、活性氧及钙离子浓度的影响  

Effects of resveratrol on cell morphology,mitochondrial membrane potential,reactive oxygen species,and intracellular calcium concentration in SGC-7901

作　　者：张秀娟[1,2] 包钰涵[1,2] 贾绍华[3] 季宇彬[1,2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心生物安全评价研究所,黑龙江哈尔滨150076 [2]国家教育部抗肿瘤天然药物教育部工程研究中心,黑龙江哈尔滨150076 [3]哈尔滨商业大学药学院,黑龙江哈尔滨150076

出　　处：《药物评价研究》2012年第3期161-164,共4页Drug Evaluation Research

基　　金：黑龙江省自然科学基金资助项目(D201043)

摘　　要：目的研究白藜芦醇对人胃癌SGC-7901细胞的影响。方法 SGC-7901细胞体外培养48h,分为白藜芦醇低、中、高剂量组(44、88、176μmol/L),阳性对照组(5-FU153.8μmol/L);阴性对照组(不含药物同体积培养液),荧光显微镜观察不同浓度的白藜芦醇对SGC-7901细胞的形态学影响;流式细胞仪检测白藜芦醇对肿瘤细胞中线粒体膜电位、活性氧的的影响;激光共聚焦显微镜观察白藜芦醇对肿瘤细胞中钙离子浓度的影响。结果显微镜下可见肿瘤细胞染色程度加深,染色质聚集、断裂,产生大小不等的凋亡小体,且随着白藜芦醇浓度加大,现象越来越明显,表明细胞凋亡的比例不断增加;白藜芦醇能够明显降低肿瘤细胞中线粒体膜电位,随着白藜芦醇浓度不断增加,肿瘤细胞中活性氧也不断增加,说明白藜芦醇能够提高肿瘤细胞中的活性氧水平来诱导其凋亡;白藜芦醇对肿瘤细胞中钙离子的浓度有一定的作用,其中高剂量能够显著提高肿瘤细胞中钙离子的浓度,且呈现一定的剂量依赖关系。结论白藜芦醇通过影响SGC-7901肿瘤细胞线粒体膜电位、活性氧及钙离子浓度导致肿瘤细胞凋亡,且与剂量相关。Objective To investigate the effect ofresveratrol on human gastric cancer SGC-7901 cell line. Methods SGC-7901 cell line was cultured in vitro for 48 h. The end concentration ofresveratrol low-, mid-, and high-dose groups was 44, 88, and 176 μmol/L, respectively; The end concentration of positive control group （5-FU） was 153.8μmaol/L; Negative control group was administrated with the same volume of culture medium without drugs. Morphology of tumor cells treated with different concentration of resveratrol was identified by fluorescence microscope. Flow cytometry was used to detect the influence of resveratrol on the changes of mitochondrial membrane potential （MMP） and reactive oxygen species （ROS） in tumor cells. After the tumor cell was exposed to resveratrol, the intracellular calcium concentration （[Ca^2＋]i） was measured with laser scanning confocal microscope. Results According to the morphology, the tumor cell staining was deepened; Chromatin aggregation and fragmentation were observed, and the apoptosis bodies were generated. With increasing the concentration of resveratrol, the phenomenon was more obvious, which indicated that the proportion of apoptosis increased. Resveratrol could reduce MMP of tumor cells, and ROS of tumor cells has also increasedwith increasing concentration of resveratrol. It indicated that resveratrol could induce tumor cell apoptosis by affecting the content of ROS. The high doses could improve [Ca^2＋]i significantly in a dose-dependent manner. Conclusion Resveratrol could induce tumor cell apoptosis by affecting MMP, ROS, and [Ca^2＋]i.

关 键 词：白藜芦醇 线粒体膜电位 活性氧 钙离子浓度 

分 类 号：R735.2[医药卫生—肿瘤]