落叶松树皮活性物质提取及红外光谱分析  

Study on Extracts of Active Substances from Larch Bark by FTIR Spectroscopy

作　　者：崔晓霞[1] 张小丽[1] 唐焕威[1] 张力平[1] 许长华[2] 孙素琴[2] 

机构地区：[1]北京林业大学材料科学与技术学院,北京100083 [2]清华大学化学系,北京100084

出　　处：《光谱学与光谱分析》2012年第7期1810-1814,共5页Spectroscopy and Spectral Analysis

基　　金：国家林业公益性行业科研专项项目(201204804);国家(973计划)重大基础研究项目(2010CB732204)资助

摘　　要：采用一维红外光谱法并结合二阶导数红外光谱法以及二维相关光谱分析技术对超声波辅助提取的落叶松树皮活性物质进行了无损快速对比分析研究。发现落叶松树皮活性物质中的主要成分是(+)-儿茶素、(-)-表儿茶素为结构单元,且以4-8位连接的二聚原花青素,没食子酸以及vitisinol为代表的一系列聚多酚化合物;二阶导数光谱中落叶松树皮原花青素在1 631,1 517,1 468cm-1处吸收峰与原花青素标准品相比,向低波数位移,而1 200和1 062cm-1处吸收峰则向高波数位移;并且在1 643,1 518,819,780,765cm-1处的吸收峰越强,(+)-儿茶素含量越高;1 518,1 469,1 112,796cm-1处的峰强则可以代表(-)-表儿茶素的含量。二维相关红外谱显示落叶松树皮活性物质谱图对角线上出现5个自动峰:1 385,1 449,1 505,1 532,1 628cm-1,以1 628cm-1最强。成功地为深入研究落叶松树皮活性物质结构鉴定提供了一种新的方法。The present study is to compare and analyze extracts of active substances from larch bark using ultrasonic wave quick- ly and undamagedly via Fourier transform infrared spectroscopy, second derivative IR spectroscopy and two dimensional spec- troscopy. In the spectra of active substances from larch bark, there are four main components as the structural unit, and （＋）- catechin, （-）-epicatechin, gallic acid and vitisinol represented a series of poly polyphenolic compounds. Furthermore the link- age between unit in proanthocyanidins dimmers was confirmed at position C（4）-C（8）. Through observing the second derivative IR spectra of active substances from larch bark, the absorption peaks, 1 631, 1 561 and 1 469 cm ^-1 of active substances moved to the smaller wave number direction, while 1 606, 1 385, 1 285 and 1 157 cm^-1 of active substances moved to the bigger wave number direction, and the intensity of characteristic peaks can represent the content of corresponding compound. In the two di- mensional spectra, the active substances from larch bark have five automatic peaks in 1 345-1 675 cm ^-1. The authors developed the new method to analyse and evaluate the active substances from larch bark successfully.

关 键 词：落叶松树皮 活性物质 原花青素 红外光谱 

分 类 号：TQ46[化学工程—制药化工]