三种致病性细菌的SERS光谱研究  

SERS Spectroscopy Study of Three Pathogenic Bacteria

作　　者：苏永波[1,2] 司民真[1,3] 张德清[3] 刘仁明[3] 林逢春 

机构地区：[1]云南师范大学物理与电子信息学院,云南昆明651000 [2]楚雄医药高等专科学校,云南楚雄675005 [3]楚雄师范学院光谱应用技术研究所,云南楚雄675000

出　　处：《光谱学与光谱分析》2012年第7期1825-1828,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(10864001,11064001)资助

摘　　要：用便携式拉曼光谱仪获得了金黄色葡萄球菌、变形杆菌、大肠杆菌在微波法制备的纳米银溶胶上的表面增强拉曼光谱。金黄色葡萄球菌在725,1 330,1 450cm-1有明显的拉曼振动峰,变形杆菌在650,725,950,1 325,1 463cm-1有明显的拉曼振动峰,大肠杆菌在650,950,1 125,1 242,1 320,1 457cm-1有明显的拉曼振动峰,对各个峰进行了初步归属。三种细菌的拉曼振动峰的位置和强度区别明显,因此SERS技术可以用于大肠杆菌、金黄色葡萄球菌和变形杆菌的快速鉴别。The SERS spectra of staphylococcus aureus, proteus, and Escherichia coli was obtained on colloidal Ag nanoparticles prepared by the microwave method with the portable Raman spectrometer. Staphylococcus aureus have obvious Raman vibrating peak at 725, 1 330 and 1 450 cm-l , proteus have obvious Raman vibrating peaks at 650, 725, 950, 1 325 and 1 463 cm-I , while E. coli have obvious Raman vibrating peaks at 650, 950, 1 125, 1 242,1 320 and 1 457 cm 1. Each peak was assigned prelimi- narily. Not only the position of Raman vibration peaks but also the intensity of the three bacteria is obviously different, so SERS can be used for identification and distinction of E. coli, staphylococcus aureus and proteus.

关 键 词：食源性疾病 SERS 大肠杆菌 金黄色葡萄球菌 变形杆菌 

分 类 号：O657.3[理学—分析化学]