制备温度对硫辛酸脂质纳米粒的影响  

Effect of Preparation Temperature on the α-Lipoic Acid Loaded Nanostructured Lipid Carriers(ALA-NLC)

作　　者：邹爱华[1] 郑锴[1] 杨晓敏[1] 夏强[2,3] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]东南大学生物科学与医学工程学院,南京210096 [3]东南大学苏州市生物医用材料与技术重点实验室,苏州215123

出　　处：《化学通报》2012年第6期519-525,共7页Chemistry

基　　金：科技部国际合作重大项目(2008DFB50060);教育部博士点新教师基金项目(200802511024);国家自然科学青年基金项目(21003047)资助

摘　　要：通过脂质和乳化剂筛选实验得到适合于硫辛酸(ALA)负载的脂质基体和乳化剂,在此基础上分别在55℃和65℃下用高压均质法(HPH)制备了硫辛酸脂质纳米粒(ALA-NLC)。通过高效液相色谱(HPLC)、动态光散射(DLS)、原子力显微镜(AFM)、微差示扫描量热(micro-DSC)研究制备温度和保存时间对ALA-NLC性质如ALA的负载量、粒径、稳定性及形貌的影响。结果表明,制备温度和保存时间对粒径、zeta电位及ALA负载量均有较大影响。Micro-DSC结果表明,55℃和65℃下制备的ALA-NLC都可能存在过冷态现象,但是55℃制备的样品更稳定,所得配方在ALA膳食补给上具有潜在的应用价值。The lipid and emulsifier of α-lipoic acid loaded nanostructured lipid carriers（ALA-NLC） were obtained by screening.ALA-NLC was prepared at two temperatures（55℃ and 65℃） by high pressure homogenization（HPH） method.The ALA load amount,particle size,stability,and morphology of the samples were investigated by HPLC,dynamic light scattering（DLS）,AFM and micro-differential scanning calorimeter（micro-DSC）.The effects of storage time and preparation temperature on the particle size,zeta potential and ALA load amount of ALA-NLC were analyzed statistically.Micro-DSC results showed that 55℃ was more appropriate than 65 ℃ for ALA-NLC preparation.However,supercooled melts might occur in ALA-NLC dispersions.The presented formulations have the potential application for ALA dietary supplement.

关 键 词：脂质纳米粒 硫辛酸 抗氧化膳食补给 

分 类 号：R943[医药卫生—药剂学]