WX-JT复方降血糖作用研究  

Study on the Hypoglycemic Effect of WX-JT Compound

作　　者：许扬[1] 刘春丽[2] 刘彪 刘颖 马勇 郑定宗 刘利波 袁勇[3] 姜大磊[4] 黄允瑜[5] 姜淼[6] 郭树仁[2] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]北大维信生物科技有限公司,北京100080 [3]青岛大学医学院附属医院,山东青岛266003 [4]青岛市市立医院,山东青岛266000 [5]北京中医药大学东直门医院,北京100700 [6]中国中医科学院中医临床基础医学研究所,北京100700

出　　处：《中国实验方剂学杂志》2012年第12期161-165,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金："重大新药创制"科技重大专项"十一五"计划(2009ZX09103-325)

摘　　要：目的:研究WX-JT复方对α-葡萄糖苷酶抑制作用,评价WX-JT复方长期给药对Ⅱ型糖尿病KK-Ay小鼠血糖相关指标影响。方法:①体外反转肠囊试验:采用大鼠小肠制备反转肠囊作为α-葡萄糖苷酶的来源,采用经α-淀粉酶消化后的淀粉溶液作为底物,与不同浓度阿卡波糖及WX-JT复方于37℃反应2 h,通过最终葡萄糖产生量计算受试药的α-葡萄糖苷酶抑制率。②正常小鼠淀粉耐量试验:正常ICR小鼠禁食20 h后单次ig给予3 g.kg-1淀粉溶液及45.24,90.48,180.96 mg.kg-1WX-JT复方,测定给予淀粉0,30,60,120 min后血糖,评价药物对血糖升高的抑制作用。③长期给药对Ⅱ型糖尿病小鼠血糖的影响:采用自发Ⅱ型糖尿病模型KK-Ay小鼠,分为模型对照组、阿卡波糖组、WX-JT复方低剂量组(180.96 mg.kg-1)和高剂量组(361.92 mg.kg-1),正常对照组采用C57 BL/6J小鼠。连续ig给药9周,定期测定空腹血糖和非空腹血糖值;试验结束前进行口服葡萄糖耐量试验(OGTT)和糖基化血红蛋白(HbA1c)测定。结果:7～189 mg.L-1WX-JT复方体外能浓度依赖地抑制α-葡萄糖苷酶活性,抑制率为16.6%～86%;90.48,180.96 mg.kg-1WX-JT复方明显降低正常小鼠淀粉耐量试验餐后30,60 min血糖;180.96,361.92 mg.kg-1WX-JT复方长期给药显著降低自发Ⅱ型糖尿病模型KK-Ay小鼠空腹血糖、非空腹血糖和糖化血红蛋白水平,改善口服葡萄糖耐量,并呈明显量效关系。结论:WX-JT复方具有明确的α-葡萄糖苷酶抑制作用,长期给药明显改善KK-Ay小鼠血糖相关指标,表明WX-JT复方通过抑制α-葡萄糖苷酶活性而发挥降血糖作用。Objective： To evaluate the inhibitory activity of WX-JT on alpha glucosidase and the effects of WX-JT （a compound Chinese medicine） on indexes associated with blood glucose in type 2 diabetic KK-Ay mice. Method： （1）Everted rat intestine experiment： normal adult Wistar rats were sacrificed and whole small intestine was removed and made into intestinal sac. The intestinal sac was placed inside the conical flask with stopper containing substrate with WX-JT and acarbose. After incubating at 37 （2） for 2 h, liquid in all conical flask was collected and the glucose level was measured. （3）In vivo evaluation of oral starch tolerance experiment. Forty ICR mice were randomly divided into 5 groups, the fasting blood glucose was measured after all mice were fasted for 12 h. Mice in WX-JT group and acarbose group were given starch solution after acute treatment at 45.24, 90.48, 180.96 mg. kg-1 WX-JT and acarbose respectively, and mice in control group were given starch solution in the same volume by gavage. The blood glucose was measured respectively at 30, 60, 120 min after oral administration. （4）All mice were divided into untreated group, acarbose group, WX-JT low （180.96 mg·kg-1） and high dose （361.92 mg·kg-1） treatment group according to FBG and weight. Ten C57 BL/6J mice were taken as normal group. Acarbose and WX-JT were given by oral administration for 9 weeks. Mice in normal group and untreated group were administered orally with sterile water in the same dosage. Fasting blood glucose was detected in every odd week and non-fasting blood glucose was detected in each even week. Oral glucose tolerance （OGTT） was detected 9 weeks later. The level of glycohemoglobin （HbAlc） was measured after all the mice were sacrificed. Result： （1）The everted rat intestine experiment showed that 7-189 mg· L-1WX-JT could inhibit the activity of alpha glucosidase enzyme in vitro in concentration-dependent manner and the inhibition rate was 16.6%-86%. （2）Oral starch tolerance experimen

关 键 词：WX—JT复方 Α-葡萄糖苷酶 II型糖尿病 

分 类 号：R285.5[医药卫生—中药学]