全雌系苦瓜ACC合成酶基因cDNA克隆及序列分析  

Cloning and Sequence Analysis of 1-Aminocyclopropane-1-Carboxylic Acid Synthase Gene cDNA from Gynoecious Momordica charantia

作　　者：王日升[1,2] 张曼[1] 方锋学[3] 黄如葵[1] 刘文君[1] 杨丽涛[4] 李杨瑞[4] 

机构地区：[1]广西农业科学院蔬菜研究所,南宁530007 [2]广西作物遗传改良生物技术重点开放实验室,南宁530007 [3]广西农业科学院甘蔗研究所,530007 [4]广西大学农学院,南宁530005

出　　处：《生物技术通报》2012年第6期54-58,共5页Biotechnology Bulletin

基　　金：国家科技支撑计划项目(2007BAD68B03);公益性行业(农业)科研专项(nyhyzx07-007-1);广西青年科学基金项目(桂科青0991077);广西自然科学基金重点项目(2010GXNSFD013031);广西作物遗传生物技术重点开放实验室开放课题(桂科能0815011-6-1-14)

摘　　要：以全雌系苦瓜‘X-Hei-d-d’花蕾为材料,根据已报道ACC合成酶(1-aminocyclopropane-1-carboxylic acid synthase,ACS)保守氨基酸序列设计简并引物,采用RT-PCR技术及序列拼接,获得了全雌系苦瓜ACS基因cDNA序列,命名为Mc-ACS4(GenBank登录号:FJ459814)。该序列包含一个1 455 bp的完整开放阅读框,编码484个氨基酸,具有7个保守区;系统进化上与普通苦瓜ACS基因首先聚类,同源性达99%,二者仅有2个氨基酸差异,推测可能与全雌系苦瓜性别分化有关。Floral buds of gynoecious bitter melon ‘X-Hei-d-d＇ were used for RNA isolation. Three cDNA fragments were amplified by two pairs of specific primers and one pair of degenerated primer designed based on the reported conserved amino acid sequence of 1-aminocyclopropane-l-carboxylic acid synthase （ ACS ） , and the full length sequence was spliced, named Mc-ACS4 （ GenBank No. FJ459814 ） . The sequence of Mc-ACS4 contained a 1455 bp open reading frame which encoded a 484 predicated amino acid and seven highly conserved region. The results of phylogenetic analysis showed that Mc-ACS4 firstly clustered with that of common bitter melon based on amino acid sequences, which shared 99% homology with only two different amino acids. It was presumed that the two different amino acids may be related with sex differentiation of gynoecious bitter melon.

关 键 词：苦瓜 全雌系 ACC合成酶 基因克隆 序列分析 

分 类 号：S642.5[农业科学—蔬菜学]