石油污染土壤中植物根际促生菌的筛选及特性分析  

Isolation and Characterization of PGPR from Petroleum-contaminated Soil

作　　者：方芳[1] 刘佳莉[1] 史煦涵[1] 陈红艳[1] 郭长虹[1] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院、分子细胞遗传与遗传育种黑龙江省重点实验室,哈尔滨150025

出　　处：《生物技术通报》2012年第6期154-158,共5页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31170479);黑龙江省自然科学基金项目(C201142);黑龙江省研究生科技创新项目(YJSCX2011-396HLJ)

摘　　要：以1-氨基环丙烷-1-羧酸(ACC)为唯一氮源,从黑龙江省大庆地区石油污染土壤的狼尾草根际土壤中分离筛选出2株产ACC脱氨酶的细菌,F4-1和F4-2。对分离的菌株进行生理生化和16S rDNA序列鉴定,确定F4-1为肠杆菌属(Enterobactersp.),F4-2为克雷伯菌属(Klebsiellasp.)。菌株F4-1的ACC脱氨酶活性为(1.40±0.17)μmolα-KA.(mg Pr.h)-1,高于F4-2的(1.03±0.03)μmolα-KA.(mg Pr.h)-1。随着L-Trp浓度的增加,菌株F4-1和F4-2的吲哚乙酸(IAA)合成量相应增加,总体上看F4-1的IAA合成能力高于F4-2。F4-1合成嗜铁素的能力也高于F4-2。We took use of the oil polluted rhizosphere soil of Pennisetum alopecuroides （ L. ） Spreng. in Daqing City in Heilongjiang province area, 1-aminocyclopropane-l-carboxylic acid as the sole nitrogen source, two plant growth-promoting bacteria strains F4-1and F4-2 which can product ACC deaminase were isolated. According to morphology and physiological biochemical characteristics and 16S rDNA, the strain F4-1 was identified as Enterobacter sp. and F4-2 was confirfned as Klebsiella sp.. The ACC deaminase activity of F4-1 was （ 1.40 ＋ 0.17 ） μmol a-KA · （mg Pr · h ） ^-1, and it was higher than F4-2 which was （ 1.03 ± 0.03 ） μmol a-KA · （ mg Pr · h ） ^-1. With the concentration of L-Trp increased, the IAA synthesis of both F4-1 and F4-2 were increased, and the IAA and sideropbore synthesis of F4-1 was higher than F4-2.

关 键 词：石油 植物根际促生菌(PGPR) ACC脱氨酶 IAA 植物修复 

分 类 号：X172[环境科学与工程—环境科学]