CCT亚基γ基因在AFB_1诱发大鼠肝癌过程中表达的变化  

Expression of CCT subunit γ in aflatoxin B1-induced hepatocarcinogenesis in Wistar rats

作　　者：欧超[1] 杨春[1] 苏建家[1] 李瑗[1] 郑海平[2] 唐艳萍[1] 李黄羿[1] 曹骥[1] 

机构地区：[1]广西肿瘤防治研究所实验研究部,南宁530021 [2]广西医科大学第一附属医院肿瘤内科

出　　处：《中国癌症防治杂志》2012年第2期136-139,共4页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

基　　金：广西自然科学基金资助项目(桂科基0832009;桂科回0991017)

摘　　要：目的探讨AFB1诱发肝癌过程中CCT亚基γ基因蛋白的表达及其意义。方法 45只Wistar大鼠随机分为AFB1组和对照组,AFB1组以200μg/kg体重腹腔注射AFB1,第13周、第33周和第53周对大鼠进行肝活检,第73周处死全部动物并取肝组织。用免疫组化法检测AFB1诱发肝癌过程中不同时期CCT亚基γ基因蛋白的表达情况。结果在第73周AFB1组CCT亚基γ基因蛋白表达水平显著高于对照组(P<0.05)。同一组不同时段比较,随着诱癌的进行,CCT亚基γ基因蛋白表达上调,第73周AFB1组CCT亚基γ基因蛋白的表达水平不仅明显高于第13周,而且亦高于第33周(P<0.01)。其余各组和同组不同时段之间CCT亚基γ基因蛋白的表达差异无统计学意义(P>0.05)。AFB1组中发生肝癌的动物肝组织CCT亚基γ基因蛋白表达率为100%(10/10),显著高于同期对照组CCT亚基γ基因蛋白的表达率30%(3/10)(P<0.05)。结论 AFB1诱癌过程中CCT亚基γ基因蛋白表达上调,CCT亚基γ基因蛋白有可能参与了肝细胞癌的发生和发展。Objective To investigate the protein expression and possible role of CCT subunit T in hepatocarcinogenesis induced by aflatoxin BI （AFBI）in rats. Methods A total of 45 Wistar rats were randomly divided into a control group and an AFB1 group,which was injected intraperitoneally with 200 ug/kg AFB1,Liver biopsies were performed on all animals at weeks 13,33 and 53.Animals were sacrificed at week 73 and liver tissues were collected.Protein expression of CCT subunit γ was dynamically examined by immunohistochemistry during different phases of AFB1-induced hepatocarcinogenesis. Results The protein expression levels of the CCT subunit γ were significantly higher in HCC tissues of the AFB1 group（ 100%, 10/10）than in tissues of the control group （30% ,3/10）. The protein level gradually increased during development of hepatic carcinoma, with the level at week 73 significantly higher than at weeks 13 and 33（P〈0.01 ）.At week 73,the level of CCT subunit γ was higher in the AFB1 group than in the control group （P〈0.05）. The expression level of CCT subunit γ did not differ significantly between the two groups at other times（P〉0.05）. Conclusion Overexpression of CCT subunit y may be related to HCC carcinogenesis.

关 键 词：肝肿瘤 CCT亚基γ基因蛋白 AFB1 大鼠肝癌 蛋白表达 

分 类 号：R735.7[医药卫生—肿瘤]