三种磷脂酶A1活力测定方法的比较  

Comparison of Three Determination Methods for Phospholipase A1 Activity

作　　者：赵梦梦[1] 薛正莲[1] 黄祖耀[1] 苏燕南[1] 马琦亚[1] 

机构地区：[1]安徽工程大学生物与化学工程学院,微生物发酵安徽省工程技术研究中心,安徽芜湖241000

出　　处：《食品与发酵科技》2012年第3期74-77,85,共5页Food and Fermentation Science & Technology

基　　金：安徽省自然科学基金项目(11040606M81);安徽省高校自然科学基金项目(KJ2009A168)

摘　　要：三种常用的磷脂酶A1活力测定的方法分别为碱滴定法、双层平板法和分光光度法。通过对磷脂酶A1标准品和实验室产磷脂酶A1菌株的活力进行测定,比较这三种方法在时间、准确性和精确性方面的差异。结果得出分光光度法具有耗时短、底物用量少、结果稳定、精确性好的优点。Three common methods of phospholipase A1 activity were alkali titration, double-plate method and spectrophotometry. The standard of phospholipase A1 and laboratory strains that produced phospholipase A1 activity were measured to compare these three methods in time, accuracy and precision differences. The results obtained that spectrophotometry possesses short time-consuming, less substrate, good stability and accuracy.

关 键 词：磷脂酶A1 活力 分光光度法 棕榈酸 

分 类 号：TQ925.6[轻工技术与工程—发酵工程]