青蒿琥酯对口腔扁平苔藓T-bet和GATA-3平衡影响的研究  

Effects of artesunate on expression of T cell-specific transcription factors T-bet and GATA-3 in oral lichen planus

作　　者：刘夏斐[1] 王燃[1] 赵玉萍[1] 范媛[1] 

机构地区：[1]南京医科大学口腔医学研究所,南京210029

出　　处：《口腔医学》2012年第6期321-324,340,共5页Stomatology

基　　金：国家自然科学基金资助项目(81170961);江苏高校优势学科建设工程资助项目(2011-2015年)

摘　　要：目的探讨青蒿琥酯(artesunate,ART)对口腔扁平苔藓(oral lichen planus,OLP)患者外周血转录因子T-bet/GATA-3失衡表达的调节作用。方法通过密度梯度离心法分离OLP患者外周血单个核细胞(peripheral blood mononuclear cells,PBMC),分别用不同浓度(0、1、100μmol/L)的青蒿琥酯和5%的碳酸氢钠溶液作用于PBMC 24 h,逆转录聚合酶链反应(RT-PCR)测定法和免疫印迹法(Western blotting)检测OLP患者PBMC中T-bet和GATA-3 mRNA和蛋白的表达。结果 T-betmRNA和蛋白的表达在100μmol/L青蒿琥酯组(0.54±0.26、0.93±0.40)均低于对照组(1.35±0.63、1.47±0.43)(P值均<0.01),GATA-3 mRNA和蛋白的表达在1μmol/L(0.62±0.33、1.11±0.69)和100μmol/L(0.23±0.21、0.44±0.27)青蒿琥酯组中均低于对照组(1.40±0.66、1.68±0.61)(P值均<0.01)。结论青蒿琥酯能够调节OLP患者T-bet和GATA-3的失衡表达。Objective To investigate the effects of artesunate on expression of the T cell-specific transcription factors T-bet/GATA-3 in oral lichen planus（OLP）.Methods Peripheral blood mononuclear cells（PBMC） were isolated by density gradient centrifugation from OLP patients.The cells were treated with different concentrations of artesunate（0,1,100 μmol/L） and 5% sodium bicarbonate for 24 hours.Expression of T-bet and GATA-3 mRNA and protein in the PBMCs was detected by reverse transcription-polymerase chain reaction（RT-PCR） and western blotting respectively.Results The expression of T-bet mRNA and protein in 100μmol/L artesunate（0.54±0.26、0.93±0.40）was weaker than that in the control group（1.35 ±0.63,1.47±0.43）（P〈0.01,P〈0.01）.The expression of GATA-3 mRNA and protein in 1μmol/L（0.62±0.33,1.11±0.69） and 100 μmol/L（0.23±0.21,0.44±0.27）artesunate was weaker significantly than that in the control group（1.40±0.66,1.68±0.61）（P〈0.01,P〈0.01,P〈0.01,P〈0.01）.Conclusions Artesunate can rectificate the imbalance of transcription factors T-bet and GATA-3.Artesunate may be an option in the treatment of OLP.

关 键 词：口腔扁平苔藓 青蒿琥酯 T-BET GATA-3 外周血 

分 类 号：R781.59[医药卫生—口腔医学]