脂多糖诱导的CD11b^＋Gr-1^＋髓源抑制性细胞对小鼠脾脏T细胞增殖的抑制作用  

Inhibition of lipopolysaccharide-induced myeloid.derived suppressor cells in the proliferation of spleen T lymphocytes

作　　者：郑燕妮[1] 于化鹏[1] 陈新[1] 邓火金[1] 樊慧珍[1] 龚雨新[1] 刘俊芳[1] 

机构地区：[1]南方医科大学珠江医院呼吸内科,广州510282

出　　处：《中华医学杂志》2012年第24期1702-1705,共4页National Medical Journal of China

基　　金：广东省科技计划项目（20108031600095）

摘　　要：目的观察脂多糖诱导的CD11b^＋Gr-1^＋髓源抑制性细胞（MDSCs）对小鼠脾脏T细胞增殖的影响，探讨其在免疫调控可能发挥的作用。方法10只6-8周龄BALB／c小鼠随机数字表法随机分为脂多糖组和对照组各5只，分别予脂多糖或生理盐水腹腔注射；采用CD11b磁珠从脾脏组织中分选MDSCs，通过瑞氏．姬姆萨染色观察细胞形态，并用流式细胞术检测细胞表面特征分子表达情况；四唑盐（MTr）比色法测定与MDSCs在体外共培养后T细胞的增殖情况。结果脂多糖组小鼠脾脏组织中CD11b^＋Gr-1^＋MDSCs比例为27．4％±6．6％，较对照组（5．1％±3．8％）明显增多（t=5．06，P=0．007）；采用CD11b磁珠从注射脂多糖的小鼠脾脏中分离出的CD11b^＋Gr-1^＋MDSCs纯度高达84．0％±4．2％；MTr法显示在MDSCs共培养后脾脏T细胞增殖水平明显低于对照组，且此作用与MDSCs的数量呈正相关（F=46．26，P=0．000）。结论CD11b磁珠可分离提取出纯度较高的脂多糖来源的MDSCs，且脂多糖诱导的MDSCs对脾脏T细胞的增殖具有抑制作用，该作用呈剂量依赖性关系。Objective To explore the effects of lipopolysaccharide （LPS） -induced myeloid-derived suppressor cells （MDSCs） on the proliferation of spleen T lymphocytes. Methods BALB/c mice were randomly divided into two groups： LPS group and normal control group. They were injected intraperitoneally with LPS and normal saline solution respectively. MDSCs were separated with CD1 lb immunomagnetic beads from the spleen extract of mice. The morphological characteristics of MDCSs were observed by Wright- Giemsa staining and the characteristic molecules on cell surface identified by flow cytometry. And the effects of MDSCs on the in vitro proliferation of T cells were determined by methylthiazolyldiphenyl-tetrazolium bromide （MTY）. Results The proportion of MDSCs in the spleen of the LPS group was much more than that of the normal control group （27.4% ±6.6% vs5.1% ±3.8%; t=5.06, P=0.007）. CD11b^＋Gr-1^＋ MDSCs could be separated by CDllb immunomagnetic beads from the spleen of mice injected with LPS at a high purity of 84.0% ±4. 2%. MTT method showed that the proliferation of T cells decreased significantly after a co-cultivation withCD11b^＋Gr-1^＋MDSCs versus the control group. And it was positively correlated with the number of MDSCs （ F = 46. 26, P = 0. 000）. Conclusions A high purity of LPS-induced myeloid-derived suppressor cells may be separated with CD11 b immunomagnetic beads. And it has dose-dependent inhibitory effects on the proliferation of the spleen T lymphocytes.

关 键 词：脂多糖 T淋巴细胞 调节性 细胞增殖 

分 类 号：R363[医药卫生—病理学]