小剂量阿托伐他汀对实验性自身免疫性重症肌无力大鼠的治疗作用  

Effects of lower dose of atorvastatin on experimental autoimmune myasthenia gravis in rats

作　　者：刘莹[1] 李晓丽[1] 徐华[2] 王杉[1] 张民[1] 李亨[1] 段瑞生[1] 

机构地区：[1]山东大学附属千佛山医院神经内科,济南250014 [2]泰安市中心医院康复科,山东泰安271000

出　　处：《山东大学学报（医学版）》2012年第7期26-31,共6页Journal of Shandong University:Health Sciences

基　　金：山东省医药卫生科研基金(2007HZ079);国家自然科学基金(30771989和81171128);教育部留学回国人员科研启动基金[教外司留(2008)890号]

摘　　要：目的探究阿托伐他汀对实验性自身免疫性重症肌无力(EAMG)大鼠的免疫调节机制。方法用人工合成的大鼠乙酰胆碱受体α亚基97-116肽段免疫Lewis大鼠制造重症肌无力(MG)模型,随机分为阿托伐他汀治疗组和对照组,采用Lennon评分标准评价大鼠病情,采用双盲法隔天评估大鼠肌力并记录体质量;免疫组化检测大鼠胸腺抑制性转录调节因子Foxp3的表达,以此反映调节性T细胞(Treg)的数量;CCK-8检测淋巴结单个核细胞增殖;ELISA检测细胞培养上清液IL-4及血清抗R97-116抗体的水平。结果阿托伐他汀治疗组大鼠临床症状较对照组明显缓解,胸腺Foxp3的表达及细胞培养上清液IL-4的水平均高于对照组,血清抗R97-116抗体水平低于对照组。结论阿托伐他汀通过上调Treg和Th2型细胞因子IL-4的水平,从而降低血清中抗R97-116抗体水平,缓解EAMG病情。Objective To investigate the immunological mechanism of atorvastatin in experimental autoimmune myas- thenia gravis （EAMG） in rats. Methods Atorvastatin was administered intraperitoneally to the EAMG rats. The intra- thymic expression of fork head box protein 3 （ Foxp3 ） was analyzed by the immunohistochemistry and the lymphocyte proliferation was determined by CCK-8. The level of IL-4 in culture supernatants of lymph node mononuclear cells （MNC） and anti-R97-116 IgG in the serum were detected by ELISA. Results Administration of atorvastatin ameliora- ted clinical signs of EAMG, increased the number of the Foxp3-positive cells in the thymic medulla, up regulated the level of IL-4 in culture supematants, and decreased the level of serum anti-R97-116 IgG. Conclusion Lower dose of atorvastatin could ameliorate EAMG by up-regulation of Treg and Th2 cytokines.

关 键 词：他汀类药物 重症肌无力 实验性自身免疫性 T淋巴细胞 白细胞介素4 大鼠 

分 类 号：R746.1[医药卫生—神经病学与精神病学]