木犀草素抑制肺癌细胞A549的增殖及其联合化疗作用  

Anti-proliferation activity of luteolin and its role in combined chemotherapy on lung cancer A549 cells

作　　者：徐统震[1] 孙雪飞[1] 任冬梅[1] 杨国涛[1] 

机构地区：[1]山东大学齐鲁医院胸外科,济南250012

出　　处：《山东大学学报（医学版）》2012年第7期50-54,共5页Journal of Shandong University:Health Sciences

基　　金：山东省科技攻关项目(2007GG20002009);山东省自然科学基金(Y2007C038)

摘　　要：目的研究木犀草素对肺癌细胞增殖的抑制及其在联合化疗中的作用。方法 MTT法检测木犀草素对A549细胞增殖的影响;碘化丙啶(PI)染色以及Annexin-V FITC和PI双参数标记后,采用流式细胞仪检测木犀草素对A549细胞周期和细胞凋亡的影响及其机制;采用JC-1染色检测细胞线粒体膜电位;划痕实验检测木犀草素对A549细胞迁移的抑制情况;采用共聚焦显微镜检测木犀草素对A549细胞应力纤维的影响,分析对细胞迁移的作用;采用细胞实时检测系统研究木犀草素和顺铂联合应用时的作用效果。结果木犀草素能够有效抑制细胞增殖,显著阻滞细胞周期、诱导细胞凋亡;细胞膜电位在木犀草素的作用下被破坏;木犀草素还能影响细胞的应力纤维进而抑制细胞的迁移,加强顺铂的抗癌作用。结论木犀草素可以通过阻断细胞周期、诱导细胞凋亡和抑制细胞的迁移发挥抗肺肿瘤作用,同时能够降低肺肿瘤细胞多药耐药性,提高肺肿瘤细胞对抗癌药物的敏感性,还可以通过影响微丝蛋白Actin的功能抑制肺癌细胞的迁移。Objective To investigate the anti-proliferation activity of luteolin in lung cancer A549 cells and to explore the effects of luteolin combined with chemotherapy. Methods Cell cycle and apoptosis of A549 cells were detected by flow cytometry with propidium iodide （PI） staining and Annexin-V. FITC and PI dual parameter markers to clarify the mechanism of luteolin on inhibiting cell proliferation. The inhibition effect of luteolin on the cell migration was detected by the mitochondrial membrane potential test and the scratch test. The effect on actin was researched through the confo- cal microscope. Chemotherapy effect of luteolin with cisplatin was detected by the cell real-time detection system. Results Luteolin could apparently inhibit cell proliferation and migration compared with the control group. Luteolin could enhance the anti-tumor effect of chemotherapy. Conclusion Luteolin can block the cell cycle in G1 phase, in- duce cell apoptosis, and inhibit cell migration. Luteolin enhances anti-tumor effect of chemotherapy.

关 键 词：木犀草素 肺癌 联合化疗 多药耐药 化疗增敏剂 

分 类 号：R734.2[医药卫生—肿瘤]