亚麻籽木酚素预防乳腺癌及与雌激素的关系  

Study of the Flaxseed Lignan on the Animal Model of Breast Cancer Prevention and the Relation with the Body Estrogen

作　　者：刘珊[1] 王小兵[1] 李爱东[2] 刘朝阳[1] 田海梅[1] 李茉[1] 赵文雅[1] 李艳芬[1] 刘静[1] 宋培培[1] 张伟[1] 

机构地区：[1]北京协和医学院,中国医学科学院,肿瘤医院,生物检测中心,北京100021 [2]北京协和医学院,中国医学科学院,肿瘤医院,中心实验室,北京100021

出　　处：《中国比较医学杂志》2012年第6期43-47,88,共6页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金资助项目(81071714)

摘　　要：目的通过卵巢切除术建立雌性大鼠去势模型,探究亚麻籽粉木酚素预防乳腺癌的功能及与雌性激素的关系。方法将48只雌性Wistar大鼠随机分为基础饲料组(BD)、基础饲料去势组(BDC)、亚麻籽粉组(FS)和亚麻籽粉去势组(FSC),每组12只,对全部大鼠进行二甲基苯蒽(DMBA)一次性灌胃(2mg/kg体重)建立诱发的乳腺癌实验动物模型;一周后对BDC组、FSC组大鼠行去势手术,连续观察21周,测定瘤体的体积和重量,并取乳腺组织进行病理学检查。结果实验期间动物一般状况良好,实验组大鼠未出现明显毒副作用;亚麻籽粉组(FS和FSC组)大鼠发生可触及肿瘤的时间较相应对照组晚2到4周;亚麻籽粉组大鼠单纯性增生和不典型增生以及乳腺癌发生率和病灶数均显著低于相应对照组(单纯性增生:FS vs BD,P=0.006**;FSC vs BDC,P<0.001**;不典型增生:FS vs BD,P=0.048*;FSC vs BDC,P=0.014*;乳腺癌:FS vs BD,P=0.028*;FSC vs BDC,P<0.047*);亚麻籽粉组大鼠肿瘤体积和重量均小于基础饲料组;FS和FSC组研究结果提示亚麻籽粉木酚素抑制增生发生及肿瘤细胞的生长的能力与实验动物体内雌性激素水平有关(单纯性增生:P=0.008**;不典型增生:P=0.042*;乳腺癌:P=0.033*)。结论亚麻籽粉木酚素可有效预防和降低化学诱癌剂DMBA所诱发的乳腺癌、癌前病变和单纯性增生的发生,预防乳腺癌的功能和效果受到体内雌性激素影响。本研究结果对未来实施木酚素预防乳腺癌及有效人群的筛选具有参考价值。Objective To explore the function of flaxseed powder lingans on the prevention of breast cancer and the relation with estrogen on the ovariectomized female rats.Methods Fouty-eight female wistar rat were randomly divided into basal diet group（BD）,basal diet castrate group（BDC）,flaxseed group（FS） and flaxseed castrate group（FSC）,All of the rats were treated with DMBA by intragastric administration at a dose of 2mg/kg weight to establish the experimental animal models of induced breast cancer and the BDC and FSC groups were operated for the ovariectomy.On the 21 week the volume and weight of the palpable tumors were measured and conducted pathological examination by pathologist.Results The experimental animals were generally in well situation,there was no obvious toxic or side effect in experimental groups,palpable tumors appeared two to four weeks later in FS and FSC groups than that in the control;Compared with the control groups,the incidence and the average number of lesions of breast hyperplasia,atypical hyperplasia,breast cancer were significantly lower in flaxseed groups（hyperplasis：FS vs BD,P=0.006＊＊;FSC vs BDC,P0.001＊＊;atypical hyperplasia：FS vs BD,p=0.048＊;FSC vs BDC,P 0.014＊;breast cancer：FS vs BD,P=0.028＊;FSC vs BDC,P=0.047＊）,additionally,the volume and weight of the tumors were lower in flaxseed groups.The results also suggested that the ability of inhibiting the hyperplasia and tumor cell growth of the flaxseed lignans is related with estrogen states（hyperplasis：P=0.008＊＊;atypical hyperplasia： P=0.042＊;Breast cancer：P=0.033＊）.Conclusions Flaxseed powder lignans can effectively prevent and reduce the occurrence of breast cancer,precancerous lesions and hyperplasia induced by DMBA,and the preventive effect of flaxseed powder lignans are affected by body estrogen.The above results may be of reference value for screening effective population and preventing breast cancer by lignans.

关 键 词：木酚素 亚麻籽粉 大鼠 乳腺癌 

分 类 号：R730.1[医药卫生—肿瘤] R737.9[医药卫生—临床医学]