黑粒小麦‘冬黑10号’的细胞学和麦谷蛋白组成分析  

Analysis of Cytology and Glutenin Composition in Black Kernel Wheat'Donghei 10'

作　　者：郭明慧[1] 裴自友[2] 温辉芹[2] 张立生[2] 程天灵[2] 李雪[2] 

机构地区：[1]山西省农业科学院食用菌研究所,太原030031 [2]山西省农业科学院作物科学研究所,太原030032

出　　处：《农学学报》2012年第6期27-30,48,共5页Journal of Agriculture

基　　金：山西省回国留学人员科研资助项目"小麦优质抗病分子标记设计育种"(2011-117);山西省科技攻关项目"山西中部高产优质抗逆小麦新品种选育"(20110311001-3);山西省农业科学院科研项目"小麦优质高产广适多抗系列新品种选育"(11yzgc042)

摘　　要：山西省作为中国优质小麦重要产区,目前已育成6个黑粒小麦品种,然而对这些黑粒小麦的染色体构成、蛋白组分却研究较少。因此,了解其遗传组成对选育黑粒小麦新品种具有指导作用。利用染色体C-分带,减数分裂中期Ⅰ染色体配对构型、基因组原位杂交结合谷蛋白亚基分析,对黑粒小麦‘冬黑10号’的染色体构成进行系统鉴定。结果表明:‘冬黑10号’体细胞染色体数目为2n=42,染色体带型与‘中国春’染色体带型未见明显变化,基因组原位杂交也未检出赖草染色体;其PMCMⅠ染色体平均构型为21Ⅱ,具有良好的细胞学稳定性,与‘中国春’小麦杂交F1的PMCMⅠ染色体构型为21Ⅱ,说明其为六倍体普通小麦。SDS-PAGE分析揭示其高分子量麦谷蛋白亚基组成为Null/7+9/5+10,低分子量麦谷蛋白亚基与对照‘中国春’不同。Shanxi Province was a important producing area of quality wheat in China, had been developed and approved six black kernel wheat varieties by Shanxi Crop Variety Approval Committee, however, their chromosome and protein constitutions of these cuhivars were less reported. Understanding its genetic composition would provided useful information for breeding. In this paper, chromosome configuration of black kernel wheat Donghei 10 was characterized by chromosome C-banding, genomic in situ hybridization, chromosome pairing configuration and glutenin subunit analysis. The results showed that the chromosomes numbered 42 （2n= 42） in its root tip-cells, chromosome C-banding patterns no significant changes from Chinese Spring, in the genomic in situ hybridization were not detected Leymus secalinus chromosomes. The mean chromosome pairing configuration was 2n= 21 II at PMC M 1, indicating Donghei 10 with good stability of cytology, and F1 hybrids with Chinese spring PMC M I chromosome configuration of 21 II showed to be hexaploid common wheat. SDS-PAGE analysis revealed that HMW-GS composed of Null/7 ＋ 9/5 ＋ 10, in addition to LMW-GS differing from that of Chinese Spring.

关 键 词：黑粒小麦 C-分带 原位杂交 高分子量麦谷蛋白亚基 低分子量麦谷蛋白亚基 

分 类 号：S512.1[农业科学—作物学]