野生口蘑菌种分离及人工驯化  

Separation and Artificial Domestication of Wild Koumo Mushroom(Tricholoma gambosum)

作　　者：修翠娟[1] 

机构地区：[1]辽宁省微生物科学研究院,辽宁朝阳122000

出　　处：《微生物学杂志》2012年第3期98-100,共3页Journal of Microbiology

基　　金：辽宁省科技攻关项目(2008201008)

摘　　要：为了给口蘑人工驯化栽培提供依据,开展了口蘑的碳、氮源营养研究。结果表明,蔗糖和牛肉膏分别为最佳碳源和最佳氮源。经转管纯化及在不同培养基驯化培养,该菌株在PSA培养基上,生长速度快、菌丝变得较浓密。但和双孢菇的菌丝相比,无论在麦粒上还是熟料上,菌丝不够粗壮,距离出菇仍然有一定的差距。口蘑的驯化栽培难度较大,欲获得实质性的突破还在于掌握其生长规律及生态习性。温度因子是影响蒙古口蘑菌丝及子实体正常生长发育的关键因子,作为长期适应低温生态环境的结果,适当温度的摸索将是驯化栽培的关键技术。口蘑具有严格的营养要求,因而培养基的筛选至关重要。A study on carbon and nitrogen sources nutrition was inaugurated in order to provide the basis for artificial domestication cultivation of koumo mushroom （ Tricholoma gambosum）. The results showed that sucrose and beef ex- tract were the respectively optimal carbon and nitrogen source. The strain in the PSA medium grew fast and the hyphae became more densely after tube-transferring purification and domestication Cultivation in different media. However, the hyphae were not stout enough as compared with mycelium of table mushroom （Agaricus bisporus） no matter on grains of wheat or done material, therefore, it still takes time and has distance to produce mushroom. The domestication of koumo mushroom is very difficult, wishing to obtain a substantial breakthrough is to master its growth law and ecologi- cal habits. The key factor that affects the hyphae and sporogonia of Mongolian koumo mushroom to grow normally is temperature. As a result of a long-term adaptation to cryo-ecological environment, properly fumbling for temperature is the key technique of the domestication cultivation. The koumo mushroom has a strict nutritional requirement, there- fore, the screening of media is of vital importance.

关 键 词：口蘑 人工驯化 转管 

分 类 号：S502.2[农业科学—作物学]