胆固醇衍生物阳离子脂质体复合EGFP-IL-1Ra质粒体外转染HELA细胞系的研究  

The transgene effect of cholesterol derivative-cationic liposome EGFP-IL-1Ra plasmid complex in HELA cell line

作　　者：蔡文虹 孙保东 张宝凤[2] 成文翔[3] 岳野[3] 虎义平[3] 李金超[3] 张鹏[3] 

机构地区：[1]深圳市人民医院风湿科,广东深圳518020 [2]深圳市人民医院营养科,广东深圳518020 [3]中国科学院深圳先进技术研究院转化医学中心,广东深圳518055

出　　处：《中国当代医药》2012年第19期5-7,共3页China Modern Medicine

基　　金：广东省深圳市科技立项项目(编号:201103348)

摘　　要：目的研究胆固醇衍生物阳离子脂质体复合EGFP-IL-1Ra质粒体外转染HELA细胞系的效率,为类风湿性关节炎治疗提供新的选择。方法制备新型胆固醇衍生物脂质体及EGFP-IL-1Ra质粒,两者结合转染HELA细胞系,通过体外凝胶阻滞试验检测阳离子脂质体与质粒DNA的最佳配比关系,MTT法检测脂质体细胞毒性,荧光免疫法检测HELA细胞的转染效率。结果实验中所用脂质体的IC50为650μg/mL,较对照组有更低的细胞毒性。脂质体/DNA质量比为50∶1作为最适比例,荧光显微镜观测脂质体结合EGFP-IL-1Ra质粒转染HELA荧光强度高于单纯EGFP-IL-1Ra质粒转染组。结论胆固醇衍生物阳离子脂质体复合EGFP-IL-1Ra质粒可高效的转染HELA细胞系,有望成为类风湿性关节炎治疗的新方法。Objective To observe the transgene effect of cholesterol derived cation liposome combine EGFP-IL-1Ra plas- mid in HELA cell line, exploring new therapeutic methods for the treatment of rheumatoid arthritis. Methods Cholesterol derived cation liposome as well as EGFP-IL-1Ra plasmid were prepared and combined to transfer the HELA cell line. Gel retarding was performed to select the appropriate liposome/DNA ratio, MTT was used to observe the cell toxicity of this complex.The transfer efficiency was observed using fluorescence microscope. Results The IC50 of theliposome was 650 μg/mL with less cell toxicity compared with the control. The 50：1 was the best liposome/NDA ratio for transferation and the cholesterol derived cation liposome complex group inidicated higher fluorescence intensity compared with the EGFP-IL- IRa loposome group. Conclusion Cholesterol derived cation liposome combine EGFP-IL-1Ra plasmid can tranfer the HELA cell line with high efficiency, which has potential therapeutic effect on RA.

关 键 词：胆固醇衍生物 阳离子脂质体 HELA细胞系 类风湿性关节炎 

分 类 号：R-33[医药卫生]