腺病毒载体介导IL-24基因对肺癌细胞的化疗增敏效应  

Recombinant adenovirus expressing IL-24 enhances the sensitivity of lung cancer cell to chemotherapeutic drugs

作　　者：李畅[1] 赵军[1] 李印[2] 梁光辉[2] 盛伟华[3] 杨吉成[3] 

机构地区：[1]苏州大学附属第一医院心胸外科,江苏苏州215006 [2]河南省肿瘤医院胸外科,河南郑州450052 [3]苏州大学医学部基础医学与生物科学学院细胞与分子生物学教研室,江苏苏州215123

出　　处：《苏州大学学报（医学版）》2012年第3期322-325,共4页Suzhou University Journal of Medical Science

基　　金：江苏省"六大人才高峰"第八批资助项目计划

摘　　要：目的研究携带人IL-24基因腺病毒载体(Ad-IL-24)联用顺铂(DDP)对人非小细胞肺癌NCI-H460细胞生长和凋亡的影响。方法取对数生长期的NCI-H460细胞,MTT比色法检测Ad-IL-24联合DDP对细胞生长的影响,流式细胞仪分析细胞周期及检测细胞凋亡率。结果 Ad-IL-24联合DDP对NCI-H460细胞的生长抑制作用和诱导凋亡效应均明显优于单用Ad-IL-24和单用化疗药物DDP,且G2/M期细胞比例显著增加(P<0.05或<0.01)。结论 Ad-IL-24能提高NCI-H460肺癌细胞对化疗药物DDP的敏感性。Objective To investigate the effects of recombinant adenovirus expressing IL-24 （Ad-IL- 24） combined with cisplatin（DDP） on growth and apoptosis of human non-small cell lung cancer NCI-H460 cells. Methods NCI-H460 cells in logarithmic phase were prepared. Effects of Ad-IL-24 combined with DDP on cell growth of NCI-H460 cells were detected by MTI＂ assay. Cell cycle and apoptosis were analyzed by flow cytometry. Results Compared with any single group, the inhibition of cell gro^h and cell apoptosis in com- bined group are more effective. Furthermore, Ad-IL-24 combined with DDP significantly increased the ratio of cells in G2/M phase（P 〈0.05 or 〈0.01）. Conclusion Recombinant adenovirus expressing IL-24 could en- hance the sensitivity of lung cancer cells to chemotherapeutic drugs DDP.

关 键 词：腺病毒 人白细胞介素-24 肺癌 基因治疗 顺铂 

分 类 号：R734.2[医药卫生—肿瘤]