PGC-1α在炎症性肠病肠黏膜组织中的表达及临床意义  

Clinical significance of expression of peroxisome proliferator-activated receptor γ coactivator 1α in the inflamed mucosa of patients with inflammatory bowel disease

作　　者：陈腾飞[1] 邬瑞金[1] 刘嫦钦[1] 刘占举[1] 

机构地区：[1]同济大学附属第十人民医院胃肠内科,上海市200072

出　　处：《世界华人消化杂志》2012年第15期1366-1370,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30971358;No.81061120521~~

摘　　要：目的:检测过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC-1α)在炎症性肠病(inflammatory bowel disease,IBD)患者肠黏膜的表达水平,探讨其在IBD患者肠黏膜组织中的作用.方法:收集15例溃疡性结肠炎(Ulcerative colitis,UC)患者、17例克罗恩病(Crohn's disease,CD)患者炎性肠黏膜活检标本及14例正常对照者内镜肠黏膜标本,采用免疫组织化学染色技术分析PGC-1α蛋白在肠黏膜中的原位表达,荧光定量PCR技术检测肠黏膜内PGC-1αmRNA的表达水平.结果:免疫组织化学分析显示:PGC-1α蛋白在正常肠黏膜上皮细胞内表达较多,黏膜固有层细胞内表达较少.与正常对照组相比,PGC-1α蛋白在UC患者肠黏膜上皮细胞内表达量明显减少,而肠黏膜固有层细胞内表达增加,PGC-1α蛋白在CD患者肠黏膜组织内表达量无明显差异.荧光定量PCR分析显示:UC患者炎症肠黏膜组织内PGC-1αmRNA表达水平显著低于正常对照组(0.48±0.15vs1.59±0.38,P<0.05),CD患者炎症肠黏膜组织内PGC-1αmRNA表达水平与正常对照组相比差异无统计学意义(1.55±0.47vs1.59±0.38,P>0.05).结论:与正常对照组相比,PGC-1α在UC炎症肠黏膜组织内表达水平降低,而在CD炎症肠黏膜中表达无明显差异,提示PGC-1α可能参与了UC发生发展过程.AIM：To investigate the expression of peroxisome proliferator-activated receptor γcoactivator（PGC） 1α in the inflamed mucosa of patients with in ammatory bowel disease（IBD）.METHODS：Inflamed colonic mucosal biopsies were collected from 15 patients with ulcerative colitis（UC）,17 patients with Crohn＇s disease（CD） and 14 healthy subjects.Expression of PGC-1α mRNA and protein in the intestinal mucosa was detected by real-time PCR and immunohistochemistry,respectively.RESULTS：Immunohistochemical analysis revealed that PGC-1α was mainly expressed in intestinal epithelial cells in healthy mucosa and lowly expressed in lamina propria mononuclearcells.The positive rate of PGC-1α protein expression in the intestinal mucosa of UC patients was significantly lower than that in healthy controls（P 0.05）,but no significant difference was found between CD patients and healthy controls（P 0.05）.Compared with healthy controls,the levels of PGC-1α mRNA were significantly decreased in the inflamed mucosa of UC patients（0.48 ± 0.15 vs 1.59 ± 0.38,P 0.05）,but not in CD patients.CONCLUSION：Aberrant expression of PGC-1α may play an important role in the pathogenesis of UC.The induction of biological effect of PGC1α may have a therapeutic role in the treatment of UC.

关 键 词：炎症性肠病 溃疡性结肠炎 克罗恩病 过氧化物酶体增殖物激活受体γ辅激活因子1α 

分 类 号：R574[医药卫生—消化系统]