MicroRNA在直肠癌中的表达谱  

MiRNA expression profiling of rectal adenocarcinoma

作　　者：屈昌民[1] 梁淑文[1] 李连勇[1] 曹艳菊[1] 王晓英[1] 翟静妤[1] 宫淑娟[1] 刘庆森[2] 

机构地区：[1]中国人民解放军第306医院消化内科,北京市100101 [2]中国人民解放军总医院消化内科,北京市100853

出　　处：《世界华人消化杂志》2012年第16期1407-1414,共8页World Chinese Journal of Digestology

摘　　要：目的:研究直肠癌microRNA(miRNA)表达谱,以期发现直肠癌发生过程和正常直肠黏膜组织的miRNA差异表达谱,并理论上预测可能受其调控的靶基因.方法:选择2011-01/03于中国人民解放军第306医院肠镜检查的进展期直肠癌患者,对直肠癌及正常组织进行miRNA芯片检测,筛选直肠癌、正常直肠组织差异表达的miRNA,并运用Gomir分析软件筛选TargetScan、PicTar、miRanda3个预测软件同时预测出的靶基因.结果:选择直肠癌组织、正常直肠黏膜组织标本各2例,通过Affymetrix miRNAs芯片检测,发现直肠腺癌与正常直肠黏膜相比,上调的miRNA为miR-15a、miR-18a、miR-18a*、miR-21、miR-24-2*、miR-27a等37种,下调的为miR-139-5p、miR-139-3p、miR-215、miR-200b、miR-133a、miR-150等18种.依据Ratio值,选择其中最高者miR-31和最低者miR-375进行预测其靶基因.预测ZFHX4可能为miR-375的靶基因,预测BACH2、FZD3等可能为miR-31的靶基因.结论:直肠癌和正常直肠黏膜相比有其特异的miRNA表达谱,miRNA在直肠癌的发生中可能起到重要作用,受其调控的靶基因及作用方式值得进一步研究.AIM： To compare the expression profile of micro-RNA （miRNA） between rectal adenocarcinoma and normal rectal mucosal tissue to identify genes possibly associated with the development of rectal cancer. METHODS： MiRNA expression profiling of two rectal adenocarcinoma samples and two normal rectal tissue samples was performed using miR-NA microarray. The possible targets of miRNAs were predicated using bioinformatic tools. RESULTS： Fifty-five miRNAs were found to be differentially expressed between rectal adenocarcinoma and normal tissues, with thirty-seven miRNAs up-regulated while eighteen down-regulated. Bioinformatic analyses indicated that ZFHX4 may be the target of miR-375, and that BACH2 and FZD3 may be the targets of miR-31. CONCLUSION： There is a specific miRNA expression profile in rectal adenocarcinoma. MiR-NAs play an important role in the development of rectal adenocarcinoma. Target mRNAs were primarily identified and worthy of further study.

关 键 词：直肠癌 微小RNA 芯片 表达谱 

分 类 号：R735.37[医药卫生—肿瘤]