miR-155在肝细胞癌中的表达及对肝癌细胞增殖的影响  

Expression of miR-155 in primary hepatocellular carcinoma and its effect on tumor cell proliferation

作　　者：胡蓉环[1] 蔡婧[1] 兰琼玉[1] 

机构地区：[1]南昌大学第二附属医院肿瘤科,江西省南昌市330006

出　　处：《世界华人消化杂志》2012年第19期1737-1741,共5页World Chinese Journal of Digestology

摘　　要：目的:检测微小RNA-155(miR-155)在肝癌组织中的表达并分析其对肝癌细胞增殖和细胞凋亡的影响.方法:采用TagMan MGB探针法荧光定量P C R分析42例原发性肝癌及对应的癌旁组织miR-155的表达;利用miR-155反义寡核苷酸(ASO-miR-155)降低肝癌细胞HepG2和SMMC7721中miR-155的表达;利用MTT比色法检测肝癌细胞增殖的变化,并通过流式细胞技术检测肝癌细胞早期凋亡情况.结果:42例肝癌及癌旁组织标本中,miR-155在52%(22/42)肝癌组织中的表达明显高于癌旁组织(P<0.05);利用脂质体将ASO-miR-155转染肝癌细胞HepG2和SMMC7721后,miR-155的表达明显降低,肝癌细胞HepG2和SMMC7721生长受到明显抑制;并且细胞的早期凋亡明显增加.结论:miR-155在肝癌组织中过表达,降低其表达能明显抑制肝癌细胞的生长并诱导细胞早期凋亡,miR-155有可能成为肝癌治疗的新靶点.AIM： To detect the expression of microRNA 155 （miR-155） in hepatocellular carcinoma and to analyze its influence on tumor cell proliferation and apoptosis. METHODS： The expression of miR-155 in 42 cases of primary liver cancer and matched tumor-adjacent normal tissue was detected by quantitative PCR. The expression of miR-155 in hepatoceUular carcinoma cell lines HepG2 and SMMC7721 was inhibited using a miR-155-spe- cific antisense oligonucleotide （ASO-miR-155）, and cell proliferation and early apoptosis were then determined by MTT assay and flow cytom- etry, respectively. RESULTS： The positive rate of miR-155 expres- sion was significantly higher in hepatocellular carcinoma （52%） than in tumor-adjacent tissue （P 〈 0.05）. After ASO-miR-155 was transfected into HepG2 and SMMC7721 cells using Lipo- fectamine, the expression of miR-155 was signifi- cantly reduced. MiR155 knockdown significantly inhibited growth and promoted early apoptosis of HepG2 and SMMC7721 cells. CONCLUSION： MiR-155 is overexpressed in hepatocellular carcinoma. Inhibition of miR-155 expression could inhibit growth and induce ear- ly apoptosis of hepatocellular carcinoma cells. MiR-155 may become a new target for the treat- ment of liver cancer.

关 键 词：原发性肝细胞癌 MIR-155 增殖 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]