HCV不同标志物检测结果比较分析  

Comparative Study on the Results Detected by Different Markers of Hepatitis C Virus

作　　者：李华[1] 龙润乡[1] 杨蓉[1] 蒋蕊鞠[1] 董承红[1] 易红昆[1] 白慧珠[1] 谢忠平[1] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所/云南省重大传染病疫苗研究重点实验室,昆明650118

出　　处：《医学研究杂志》2012年第6期17-20,共4页Journal of Medical Research

基　　金：国家高技术发展计划("863"项目)基金资助项目(2007AA02Z400);云南省联合资助项目计划(2008GA008)

摘　　要：目的分析丙型肝炎病毒抗原、抗体及核酸标志物实验室检测结果之间的关联性。方法用丙型肝炎病毒抗体(HCV-Ab)、丙型肝炎病毒核酸(HCV-RNA)扩增(PCR)荧光定量、丙型肝炎病毒核心抗原(HCV-CAg)及丙型肝炎病毒抗原(HCV-Ag)检测试剂盒,分别检测血清或血浆样品中的HCV-Ab、HCV-RNA、HCV-CAg和HCV-Ag丙型肝炎病毒4种标志物,对检测结果之间的关联性进行分析。结果在1551份血清或血浆样品中,共检出HCV-Ab阳性样品565份(36.43%)、HCV-Ag(游离抗原)阳性样品48份(3.09%)、HCV-RNA阳性样品317份(20.44%)、HCV-CAg阳性样品25份(1.61%),HCV-Ab及HCV-RNA检出率明显高于抗原检出率(P<0.01),其阳性样品检出率按高低顺序依次为HCV-Ab、HCV-RNA、HCV-Ag和HCV-CAg。结论 4种标志物的检测可以联合运用,能有效降低HCV-Ab检测带来的漏检风险。Objective To analyze the relevance of results detected by HCV - CAg, HCV - Ab, HCV - RNA as markers. Methods The enzyme immunoassay kits were used to measure the level of HCV - Ab, HCV - RNA, HCV - CAg and HCV - Ag in serum or plasma samples, and the correlation among them was evaluated by statistic analysis. Results The positive of HCV - Ab, HCV - RNA, HCV - CAg and HCV - Ag were 565, 317,48,25among the 1551 clinical serum and plasma samples, respectively. The detection rate of HCV - Ab and HCV - RNA was obviously higher than HCV - CAg（P 〈0.01 ）. The positive rate was in sequence of HCV - Ab 〉 HCV - RNA 〉 HCV - CAg 〉 HCV - Ag. Conclusion The united application of different four markers can reduce the undetected probability efficiently.

关 键 词：丙型肝炎病毒 标志物 HCV—Ab HCV—RNA HCV—CAg HCV—Ag 

分 类 号：R[医药卫生]