蔡氏扶正消癥汤联合奥沙利铂抑制胃癌生长的研究  

Cai′s-fu-zheng-xiao-zheng Decoction Potentiates Antitumor Activity of Oxaliplatin in Gastric Cancer in vitro and in vivo

作　　者：王海波[1] 蔡慎初[1] 徐晓峰[1] 吴式琇[1] 黄庆科[1] 

机构地区：[1]浙江省温州医学院附属第一医院,325000

出　　处：《医学研究杂志》2012年第6期74-77,共4页Journal of Medical Research

基　　金：浙江省中医药管理局基金资助项目(2009CA074)

摘　　要：目的探讨蔡氏扶正消癥汤联合奥沙利铂对体内外胃癌生长的影响及其机制。方法奥沙利铂、蔡氏扶正消癥汤及联合用药作用人胃癌细胞株SGC-7901后,CCK-8法检测细胞增殖;流式细胞术检测细胞凋亡;Western blotting检测胃癌细胞中Bax和Bcl-2的表达;建立裸鼠胃癌皮下移植瘤模型,分别给予奥沙利铂、蔡氏扶正消癥汤及联合治疗,实验结束后分别测量各组裸鼠皮下移植瘤重量后计算抑瘤率;免疫组织化学法检测各组裸鼠肿瘤组织中Bax和Bcl-2的表达。结果蔡氏扶正消癥汤可明显增强奥沙利铂对体外胃癌细胞的增殖抑制和凋亡诱导作用;同时蔡氏扶正消癥汤联合奥沙利铂可显著抑制裸鼠胃癌皮下移植瘤生长,与对照组和奥沙利铂组相比较均有显著性差异(P<0.05);蔡氏扶正消癥汤联合奥沙利铂可分别上调和下调Bax和Bcl-2在体内外胃癌中的表达。结论蔡氏扶正消癥汤可增强奥沙利铂对体内外胃癌生长的抑制作用,该作用可能通过上调Bax表达和下调Bcl-2表达而实现。Objective To investigate the effect and the mechanism of cai＇ s - fu - zheng - xiao - zheng decoction in the growth inhi- bition of gastric cancer by oxaliplatin. Methods After human gastric cancer cell line SGC - 7901 was treated with cai＇ s - fu - zheng - xiao -zheng decoction, oxaliplatin and cai＇s -fu -zheng- xiao- zheng decoction combined oxaliplatin, the cellular proliferation was de- tected by Cell Counting Kit- 8 （CCK- 8） assay. The flow cytometry （FCM） was used to determine apoptosis in gastric cancer ceils. Western blotting was used to detect the protein expression of Bax and Bcl -2. SGC -7901 cells were injected subcutaneously into nude mice to establish gastric xenograft tumors. Tumor weight and inhibition rate were evaluated respectively after the mice were sacrificed. Im- munohistochemistry was used to detect the positive expression of Bax and Bcl -2 in the xenograft tumors. Results Cai＇s - fu - zheng - xiao - zheng decoction combined with oxaliplatin induced a higher percentage of growth inhibition and apoptosis in gastric cancer cell line SGC -7901 than that of oxaliplatin alone. The expression of Bax and Bcl - 2 were up - regulated and down - regulated, respectively in human gastric cancer SGC -7901 cells after co -treatment of cai＇s -fu -zheng- xiao -zheng decoction plus oxaliplatin. The final tumor volumors showed significant decrease in the cai＇s - fu - zheng - xiao - zheng decoction plus oxaliplatin group compared with control or with oxaliplatin alone. Compared with the control and oxaliplatin alone, being treated with combination significantly increased the expression of Bax in tumor tissues, but the positive expression of Bcl - 2 was decreased. Conclusion Cai＇s - fu - zheng - xiao - zheng decoction could potentiate the growth inhibition of gastric cancer induced by oxaliplatin, which may be related to up - regulation of Bax and down - regula- tion of Bcl -2.

关 键 词：胃癌 奥沙利铂 蔡氏扶正消癥汤 BAX BCL-2 

分 类 号：R[医药卫生]