乳腺癌组织血清NF-κB p65表达分析及临床意义  

Clinical Significance of The Expressions of NF-κB p65 In Serum and Tissues from Breast Cancer

作　　者：杨明峰[1,2] 李志国[1,2] 孙红岗[1,2] 董学君[1,2] 

机构地区：[1]浙江省绍兴市人民医院 [2]浙江大学绍兴医院分子医学中心,312000

出　　处：《医学研究杂志》2012年第6期78-81,共4页Journal of Medical Research

基　　金：浙江省医药卫生科学研究基金资助项目(2009A208);浙江省科技厅公益类项目(2010C33010);绍兴市科技计划项目(2009A23005)

摘　　要：目的检测乳腺癌患者血清和组织NF-κB p65的表达,探讨NF-κB p65血清与组织表达是否一致;血清表达与乳腺癌发生发展的关系。方法采用RT-PCR和Western blotting检测41例乳腺癌及癌旁组织NF-κB p65的表达。ELISA法检测80例乳腺癌、60例正常和良性肿块患者血清NF-κB p65水平。分析血清与组织NF-κB p65表达关系及血清表达与临床病理结果的关系。结果 41例乳腺癌患者组织RT-PCR和Western blotting结果表明NF-κB p65 mRNA和蛋白表达水平均高于癌旁和良性组(P<0.05),高表达率为(87.9%,82.9%);血清NF-κB p65的阳性率为85.4%,血清和组织表达一致性检验发现两者具有统计学意义(Kappa=0.458),表达一致率为73.2%。80例乳腺癌患者血清NF-κB p65表达与病理结果相关性分析表明血清NF-κB p65表达与乳腺癌患者的淋巴结是否转移及Her-2高表达明显相关(P<0.05)。结论血清NF-κB p65浓度可反映乳腺癌患者组织NF-κB p65表达水平,同时可反应乳腺癌患者转移和Her表达情况,为临床诊断乳腺癌提供了新的思路。Objective To investigate the consistency of NF - kB p65 expression in serum and tissue, and study the relationship be- tween serum NF -kB p65 and the development of breast cancer. Methods Expressions of NF - kB p65 in breast cancer, adjacent and benign tumor tissues were detected by RT - PCR and Western blotting. Expressions of NF - kB p65 in surem were detected by ELISA. The agreement of NF - KB p65 expression in serum and tissue was analyzed by Kappa test. And the clinical significance of serum NF - kB p65 in the development of breast cancer was analyzed by investigating the relationship of the expression levels of serum NF - kB p65 with clinical pathology results. Results The frequency of NF -kB p65 mRNA positive was （87.9% ,82.9% ） by RT - PCR and Western blotting, and serum NF -kB p65 positive was 85.4% by ELISA. The statistically significant Kappa test indicated that NF - kB p65 ex- pression in tissue and serum were agreement （ Kappa = O. 458）. The levels of serum NF - kB p65 was correlative with lymph node metas- tasis, ER, PR and Her - 2 expression （P 〈 O. 05）. Concluslon The levels of serum NF - kB p65 response to that in breast cancer tis- sues. Serum NF - kB p65 may be a biomarker of breast cancer malignancy.

关 键 词：乳腺癌 转录因子 NF-ΚB 

分 类 号：R737.9[医药卫生—肿瘤]