Fcα受体在类风湿性关节炎人成纤维样滑膜细胞的表达与功能研究  

Expression of Fcα Receptor in Human Fibroblast-like Synoviocytes of Rheumatoid Arthritis and Its Function

作　　者：喻晶[1] 聂李平[1] 颜存粮[1] 刘小平[1] 桂耀庭[2] 

机构地区：[1]北京大学深圳医院检验科,深圳518036 [2]北京大学深圳医院中心实验室,深圳518036

出　　处：《医学研究杂志》2012年第6期97-99,共3页Journal of Medical Research

基　　金：深圳市科技计划基金资助项目(201103006)

摘　　要：目的探讨Fcα受体在类风湿性关节炎人成纤维样滑膜细胞的表达特征及其功能。方法采用RT-PCR和免疫荧光技术,从基因和蛋白水平检测Fcα受体在类风湿性关节炎人成纤维样滑膜细胞(RA FLS)的表达;采用ELISA方法,检测IgA对RA FLS分泌IL-6的影响。结果采用RT-PCR和免疫荧光染色方法,均检测到Fcα受体在RA FLS的表达。sIgA与RAFLS孵育12h后,细胞分泌IL-6的水平明显增高,差异具有显著性(P<0.01)。mIgA与RA FLS孵育12h后,细胞分泌IL-6的量无明显改变(P>0.05)。sIgA+TNF-α同时与RA FLS孵育12h后,细胞分泌IL-6含量明显高于sIgA或TNF-α单一组(P<0.01)。结论 Fcα受体在类风湿性关节炎人成纤维样滑膜细胞存在表达,sIgA增高RA FLS分泌IL-6的水平,并与TNF-α产生协同作用。Yu Jing, Nie Liping, Yah Cunliang , Liu Xiaoping , Gui Yaoting. Beijing University Shenzhen Hospital, Guangdong 518036, China Abstract Objective To investigate the expression and function of Fcαreceptor on human fibroblast - like synoviocytes （FLS） of rheumatoid arthritis （RA）. Methods RT - PCR and immunofluorescence technique were applied to detect the expression of Fcαreceptor on human RA FLS. The effect of IgA on the level of interleukin - 6 （ IL - 6） secreted by human RA FLS was measured by using ELISA. Results The results from RT - PCR and immunofluorescence staining confirmed the expression of Fcαreceptor on human RA FLS. Com- pared with control, secretory IgA （sIgA） increased the level of IL- 6 released by human RA FLS after 12h incubation （P 〈 0.01 ）. Mon- omeric IgA （mIgA） did not result in significant changes on the IL - 6 production of human RA FLS （ P 〉 O. 05 ）. After 12h incubation, sI- gA ＋ TNF - α increased the IL - 6 concentration dramatically, compared to sIgA or TNF - α alone （P 〈 O. O1 ）. Conclusion Fcα receptor is expressed on human RA FLS. SIgA increases the IL - 6 concentration of human RA FLS. SIgA and TNF -α together have synergistic effect on the IL -6 production.

关 键 词：人成纤维样滑膜细胞 Fcα受体 IL-6 类风湿性关节炎 

分 类 号：R[医药卫生]