盐酸氨基胍对兔牙扭转过程中牙周组织内破骨细胞的影响  

The effect of aminoguanidine on osteoclast in periodontal tissues during the rotation movement of teeth in rabbits

作　　者：孙嵘[1] 吴丽萍[2] 

机构地区：[1]苏州口腔医院正畸科,江苏苏州215005 [2]同济大学口腔医院正畸科．上海200072

出　　处：《上海口腔医学》2012年第3期251-256,共6页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30672351)~~

摘　　要：目的:研究盐酸氨基胍(AG)对兔牙扭转移动过程中牙周组织内破骨细胞的影响,并分析其机制。方法:选取36只雄性新西兰大白兔,随机分为2组,建立兔正畸牙扭转移动模型。分别于扭转牙颊侧局部骨膜下注射40mg/mL盐酸氨基胍(AG组)和生理盐水(NaCl组),隔天1次。于加力后第3、7、14、21、28和42天处死动物,取材制作切片,行TRAP酶组织化学和诱导型一氧化氮合酶(iNOS)免疫组织化学染色,观察破骨细胞募集以及iNOS的表达情况。采用SPSS16.0软件包对数据进行统计学分析。结果:2组破骨细胞募集数量变化趋势具有相似规律。与NaCl组相比,AG组自第14天起破骨细胞募集数量显著降低(P<0.05)。自加力第21天起,AG组牙周组织压力侧iNOS表达的阳性面积百分比显著小于NaCl组(P<0.05)。AG组破骨细胞计数与iNOS阳性表达面积百分比呈正相关(r=0.875,P<0.05)。结论:AG对iNOS的表达有抑制作用,可能是通过减少NO生成而间接抑制破骨细胞的募集。PURPOSE： To investigate the effect of aminoguanidine on osteoclast in periodontal tissues during experimental teeth movement in rabbits. METHODS： 36 male New Zealand white rabbits were divided randomly into two groups （18 rats in each group）. The rabbits of the two groups received subperiosteal injections of aminoguanidine （as AG group） and normal saline （as NaC1 group） locally at 2-day intervals respectively. Rabbits were sacrificed at 3,7,14,21,28,42 days after m^hodontic force application.Immunohistochemistry and TRAP enzyme stain were used to detect the expression of iNOS and observe the osteoclasts. The data was analyzed with SPSS16.0 software package. RESULTS： Collecting osteoclasts had the same change in all groups. From the 14th day till the end of the experiment, the AG group showed a significant decrease in the number of osteoclast （P〈0.05）compared with the NaC1 group. AG could effectively restrain the expression of iNOS in the pressure side after 21 days.The number of osteoclast was positively correlated with the proportion of iNOS in AG group （r=0.875,P〈0.05）. CONCLUTIONS： AG could restrain the expression of iNOS, and affect the number of osteoclast by reducing the biosynthesis of NO in rabbits

关 键 词：盐酸氨基胍 诱导型一氧化氮合酶 牙扭转 破骨细胞 

分 类 号：R783.5[医药卫生—口腔医学]