CCN2及APC蛋白在结直肠癌组织中的表达及意义  

The roles of CCN2 and APC in colorectal cancer

作　　者：孙政[1] 曹杰[1] 梁立源 杨平[1] 张通[1] 张伟健[1] 唐伟镖[1] 

机构地区：[1]广州医学院附属广州市第一人民医院胃肠外科,510180 [2]广东省从化市中心医院普通外科,510900

出　　处：《广东医学》2012年第11期1556-1559,共4页Guangdong Medical Journal

基　　金：广州市医药卫生科技项目重点资助项目(编号:2009-ZDi-08)

摘　　要：目的探讨CCN2及APC蛋白在结直肠癌组织中的表达及临床意义。方法采用免疫组化SP技术检测正常结直肠黏膜组织、癌旁组织、结直肠腺瘤、非转移性结直肠癌、转移性结直肠癌(metastatic colorectalcancer,mCRC)及远处转移癌组织各30例中CCN2蛋白及APC蛋白的表达情况;结合结直肠癌患者的临床病理参数进行分析。结果非转移性结直肠癌、mCRC及远处转移组织中CCN2蛋白的表达率分别为76.67%、73.33%和70.00%;而在癌旁组织、结直肠腺瘤和正常结直肠黏膜组织中的表达率分别为6.67%、23.33%和0.00%,差异有统计学意义(2=74.263,P=0.000);APC蛋白在非转移性结直肠癌、mCRC及远处转移组织中均表现为低表达(表达率分别为26.67%、23.33%和16.67%),在癌旁组织、结直肠腺瘤、正常结直肠黏膜组织中均表现为高表达,分别为86.67%、76.67%和90.00%,差异有统计学意义(2=70.00,P=0.000);CCN2表达与结直肠癌患者年龄、性别、肿瘤最大径和肿瘤组织病理分型无明显关系(P>0.05),而与肿瘤的TNM分期及5年生存率相关(分别为2=4.689,P=0.03;2=4.13,P=0.042);APC蛋白表达与CCN2表达呈明显负相关(rs=-0.467,P=0.001)。结论 CCN2基因是一种癌基因,CCN2在结直肠癌中表达与结直肠癌临床病理指标有关系,CCN2可考虑作为诊断结直肠癌或判断结直肠癌预后的指标;CCN2在结直肠癌中表达与APC有关,且可能受Wnt信号通路的调节。Objective To investigate the roles of CCN2 and APC in colorectal cancer. Methods Immunohisto- chemical staining of CCN2 and APC were performed in 180 samples, including each 30 samples of normal rectum mucosa tissue, adjacent noncancerous tissue, adenoma of colorectum, non -metastatic colorectal carcinoma, metastatic colorectal carcinoma and distant metastasis tissue. Results The positive rates of CCN2 in non - metastatic colorectal carcinoma, metastatic colorectal carcinoma and distant metastasis tissues were 76. 67%, 73.33% and 70. 00%, respectively, which were significantly higher than those in the mucosa tissue of normal rectum, adjacent noncancerous tissue, and adenoma of colorectum （χ^2 =70. 00, P =0. 000）. The positive rates of APC in adjacent tissue, adenoma of colorectum and mucosa tissue of normal rectum, were 86. 67%, 76. 67% and 90. 00%, respectively, which were significantly higher than those in the non- metastatic colorectal carcinoma, metastatic colorectal carcinoma and distant metastasis tissue （χ^2= 70. 00, P = 0. 000）. The expression of CCN2 was significantly correlated with the TNM neoplasm phasing and five - year survival （χ^2 = 4. 689, P = 0. 03 ; χ^2 = 4. 13, P = 0. 042）, while the expression of APC and CCN2 was significantly nega- tively correlated （r5 = -0. 467, P =0. 001 ）. Conclusion CCN2 gene is a oncogene, of which expression is correlated with the clinical pathology of colorectal cancer. The expression of CCN2 in colorectal cancer may be regulated by Wnt sig- nal pathway.

关 键 词：结直肠癌 CCN2 APC WNT信号通路 

分 类 号：R730.231[医药卫生—肿瘤]