新生鼠缺血脑组织MMP-9、TIMP1表达及其意义  

Expression and significance of MMP-9 and TIMP1 in newborn rat brain after ischemia injury

作　　者：黄越[1] 吴春[1] 刘增荣[1] 王建军[1] 

机构地区：[1]川北医学院附属医院儿科,四川南充637000

出　　处：《川北医学院学报》2012年第3期250-252,共3页Journal of North Sichuan Medical College

基　　金：四川省卫生厅科研课题资助项目(100144)

摘　　要：目的:观察新生大鼠缺血脑组织基质金属蛋白酶-9(matrix metallo proteinase system-9,MMP-9)、基质金属蛋白酶组织抑制物1(TIMP1)表达情况及其作用。方法:75只7 d龄Wistar大鼠随机分为正常对照组、缺氧缺血组(模型组)、假手术组。采用左侧大脑中动脉结扎术建立脑缺氧缺血组模型,24 h后用免疫组化法观察新生大鼠缺血脑组织MMP-9、TIMP1表达水平。结果:MMP-9、TIMP1在三组脑组织中均有表达,但组间差异有统计学意义(P<0.01);模型组缺血脑组织MMP-9、TIMP1表达水平较假手术组及对照组均显著升高(P<0.01)。其中模型组缺血脑组织MMP-9与TIMP1表达水平呈显著正相关(P<0.01);正常对照组和假手术组脑组织MMP-9与TIMP表达水平之间无显著相关性(P>0.05)。结论:MMP-9、TIMP1在新生鼠缺血脑组织表达明显增加,可能参与新生鼠缺血脑组织损伤的发生。Objective：To study the effects of the matrix metalloproteinase-9 （MMP-9） and tissue inhibitors of metalloproteinases 1 （TIMP1） in cerebral tissues of newborn rats after brain ischemia injury. Methods：Seventy-five,seven-day-old rats were classified into three groups randomly ： normal control group （ n = 25 ） , hypoxia - ischemia group （ 25 ） , and sham operation group （25）. The rats in the hypoxia-isehemia group were subjected to right carotid artery oceusion,and the cerebral MMP-9 and TIMP1 levels were detected by im- muno-histochemistry method 24 hours later. Results：MMP-9 and TIMP1 were expressed in the three groups,but there were significant differences among the three groups. The cerebral MMP-9 and TIMP1 levels were increased in the hypoxia-ischemia group compared with those in the normal control and sham operation group （ P 〈 0.01 ） , and the cerebral MMP-9 level showed positive correlation with TIMP1 level in the hypoxia - isehemia group （P 〈 0. 01 ） ,whereas no correlation was founded in the normal control or the sham opera- tion group （P 〉 0.05 ）. Conclusion：The expression of MMP-9 and TIMP1 is increased in newborn ischemic brain and may contribute to the brain injury after ischemia condition.

关 键 词：脑缺血 基质金属蛋白酶9 金属蛋白酶抑制物1 新生大鼠 

分 类 号：R741.02[医药卫生—神经病学与精神病学]