氨肽酶N／CD13在肝癌中的表达及意义  

Significance of aminopeptidase N/CD13 expression in hepatocellular carcinoma

作　　者：邱谢武[1] 于聪慧[1] 王剑飞[1] 奈超[1] 田玉旺[2] 

机构地区：[1]北京军区总医院肝胆外科,北京100700 [2]北京军区总医院病理科,北京100700

出　　处：《国际外科学杂志》2012年第6期384-387,F0003,共5页International Journal of Surgery

基　　金：首都医学发展科研基金资助项目（No.2007-3024）

摘　　要：目的探讨氨肽酶N／CD13在肝癌患者中的表达情况，探讨其与肝癌患者临床资料及预后的相关性。方法应用免疫组化SP法，对40例肝癌患者肝癌组织、10例肝癌患者癌旁组织及10例肝血管瘤患者正常肝组织行单克隆抗体CD13检测。结果40例（100％）肝癌组织均可见不同程度的CD13阳性表达，肝癌癌旁组织中3例（30％）出现CD13弱阳性表达，10例肝血管瘤患者标本未见表达。CD13表达量与患者的性别、年龄、血清AFP值、HbsAg、分化程度、Child分级及TNM分期差异无统计学意义（P〉0．05）；而CD13的表达量与患者的血清AFP值、HbsAg、分化程度之间差异有统计学意义（P〈0．05）。生存函数图发现CD13与患者生存时间呈反相关，但CD13表达量与患者肿瘤复发差异无统计学意义（P〉0．05）。结论CD13可作为肝癌干细胞的表面标志物，有望成为判断预后的有效指标。Objective To study aminopeptidase N/CD13 expression in hepatocellular carcinoma and its relationship with clinical data and prognosis in patients with hepatocellular carcinoma. Methods The immunohistoehemical SP method was used to detect the CD13 monoclonal antibody in 40 cases of hepatocellular carcinoma,10 cases of corresponding para-carcinoma and 10 cases of cavernous hemangioma. Results Forty cases （100%） hepatoeellular carcinoma tissues were seen varying degrees of CD13 expression, 3 （30%） corresponding para-carcinoma tissues were weakly positive, and 10 cases of cavernous hemangioma with no expression. The expression rate of CD13 was not significantly correlated with the patient gender, age, serum AFP value, HbsAg, differentiation, CHILD grade and TNM stage （ P 〉 0. 05 ）. But the expression of CD13 was closely related with the pa- tient serum AFP value, HbsAg, differentiation （ P 〈 0. 05）. By the survival function graph we could find the expression rate was negativly correlated with survival in patients, but the expression was not significantly correlated with tumor relapse. Conclusion CD13 can be used as the surface marker of liver cancer stem cells,and it is expected to become an effective indicator of prognosis in patients with hepatocellular carcinoma.

关 键 词：肝癌 肝癌干细胞 CD13 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]